关键词: 南非, 羊毛, 伊文斯扇头蜱, 鉴定

Abstract: Objective To quarantine and identify the ticks carried by wool imported from South Africa to China, and to prevent the transmission of harmful parasites through international trade. Methods Thirty-one batches of wool imported from South Africa at Shanghai port from January 2016 to August 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene and cytochrome C oxidase subunit I gene were amplified and sequenced for molecular biological identification. Results Thirty-one batches of wool were imported from South Africa at Shanghai port from January 2016 to August 2018. Ticks were observed in 8 batches of the imported wool, with a positive detection rate of 25.8%. They belonged to 8 species and 3 genera as identified by morphological and molecular biological methods. There were 15 ticks of Rhipicephalus evertsi evertsi, as the predominant species (53.6% of the total number) of intercepted ticks carried by the wool imported from South Africa; there was no distribution record of this tick species in China. Conclusion The intercepted ticks carried by wool imported from South Africa are mainly Rh. e. evertsi, which can transmit multiple animal diseases including babesiosis.

Key words: South Africa, Wool, Rhipicephalus evertsi evertsi, Identification