红带锥蝽的实验室人工饲养和部分生物学特性观察

doi:10.11853/j.issn.1003.8280.2019.03.012

中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (3): 281-285.DOI: 10.11853/j.issn.1003.8280.2019.03.012

红带锥蝽的实验室人工饲养和部分生物学特性观察

朱丹, 刘琴, 张仪, 李元元, 吴嘉彤

中国疾病预防控制中心寄生虫病预防控制所, 卫生部寄生虫病原与媒介生物学重点实验室, 世界卫生组织热带病合作中心, 国家热带病中心, 上海 200025

收稿日期:2018-12-28 出版日期:2019-06-20 发布日期:2019-06-20
通讯作者: 刘琴,Email:liuqin@nipd.chinacdc.cn
作者简介:朱丹,男,主任技师,主要从事医学媒介生物防控研究,Email:zhudan@nipd.chinacdc.cn
基金资助:
国家重点研发计划（2016YFC1202000， 2016YFC1202002）；国家科技重大专项（2018ZX10101002-005）

Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata

ZHU Dan, LIU Qin, ZHANG Yi, LI Yuan-yuan, WU Jia-tong

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Chinese Center for Tropical Diseases Research, WHO Collaborating Centre for Tropical Diseases, National Center for International Research on Tropical Diseases, Ministry of Science and Technology, Key Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai 200025, China

Received:2018-12-28 Online:2019-06-20 Published:2019-06-20
Supported by:
Supported by the National Key Research and Development Plan (No. 2016YFC1202000, 2016YFC1202002) and National Science and Technology Major Project of China (No. 2018ZX10101002-005)

摘要/Abstract

摘要：

目的建立一套规范完整的实验室红带锥蝽人工饲养技术和方法，为了解红带锥蝽的生活史、分子生物学特性、锥蝽传播疾病及防控的研究提供参考资料。方法在温度（28±1）℃，相对湿度（70 ±5）%的恒温恒湿培养箱中12 h光照和12 h黑暗条件下，以昆明鼠为唯一饲血动物，对红带锥蝽进行实验室人工饲养。结果红带锥蝽在人工饲养环境下可完成整个生活史的发育。红带锥蝽完成一个世代需126～153 d，Ⅰ～Ⅴ龄期若蝽、成蝽各期均在鼠体嗜血。结论通过实验室人工饲养，成功进行了红带锥蝽的繁殖。

关键词: 红带锥蝽, 人工饲养, 生物学特性

Abstract:

Objective To establish a set of standard techniques and methods for laboratory rearing of Triatoma rubrofasciata, and to provide a reference for understanding the life cycle and molecular biological characteristics of T. rubrofasciata, the diseases transmitted by T. rubrofasciata, and the prevention and control of these diseases. Methods Triatoma rubrofasciatawas artificially fed in an incubator with a constant temperature of (28±1)℃ and a relative humidity of (70±5)% under the laboratory condition of light for 12 hours and darkness for 12 hours. Kunming rats were used as the sole blood-feeding animal. Results Triatoma rubrofasciatacompleted the whole life cycle (126-153 days) under the artificial rearing condition. Stage I-V nymphs and adults all were fed with the blood of rats. Conclusion The reproduction of T. rubrofasciata is successfully carried out by laboratory rearing.

Key words: Triatoma rubrofasciata, Laboratory rearing, Biological characteristics

中图分类号:

R384

朱丹, 刘琴, 张仪, 李元元, 吴嘉彤. 红带锥蝽的实验室人工饲养和部分生物学特性观察[J]. 中国媒介生物学及控制杂志, 2019, 30(3): 281-285.

ZHU Dan, LIU Qin, ZHANG Yi, LI Yuan-yuan, WU Jia-tong. Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata[J]. Chines Journal of Vector Biology and Control, 2019, 30(3): 281-285.

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红带锥蝽的实验室人工饲养和部分生物学特性观察

Laboratory rearing and observation on the biological characteristics of Triatoma rubrofasciata

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