组学方法在昆虫杀虫剂抗性机制研究中的应用

doi:10.11853/j.issn.1003.8280.2018.03.027

摘要/Abstract

摘要： 长期以来杀虫剂在卫生和农业害虫防治领域的广泛使用，使昆虫杀虫剂抗性的产生成为进化的一种必然结果，昆虫杀虫剂抗性的防控和新型杀虫剂的研究均有赖于对杀虫剂抗性机制的研究。近年来，随着基因组学、转录组学、蛋白质组学和代谢组学的发展和应用，昆虫对杀虫剂抗性机制的研究已经取得较多新进展。该文对组学方法在昆虫杀虫剂抗性机制研究中的应用作一综述，并对组学研究在昆虫杀虫剂抗性研究中的意义和应用前景进行总结与展望。

关键词: 昆虫, 抗药性, 组学

Abstract: The widespread use of insecticides in the field of pest control in public health and agriculture has led to the emergence of insecticide resistance as an inevitable result of evolution. Insecticide resistance management and the development of new insecticides all rely on the research on the mechanism of insecticide resistance. With the development and application of new technologies, such as genomics, transcriptomics, proteomics and metabolomics, many new advances have been made in insecticide resistance mechanisms. In this article, the application of related omics approaches in insecticide resistance research was reviewed, and the significance and application prospect of omics study in insecticide resistance research were summarized.

Key words: Insect, Insecticide resistance, Omics

中图分类号:

卫沫, 唐建霞, 朱国鼎, 曹俊. 组学方法在昆虫杀虫剂抗性机制研究中的应用[J]. 中国媒介生物学及控制杂志, 2018, 29(3): 317-320.

WEI Mo, TANG Jian-xia, ZHU Guo-ding, CAO Jun. Application of omics approaches in insecticide resistance mechanism research[J]. Chines Journal of Vector Biology and Control, 2018, 29(3): 317-320.

参考文献

[1] Denholm I, Devine GJ, Williamson MS. Insecticide resistance on the move[J]. Science, 2002, 297(5590):2222-2223.
[2] WHO Expert Committee on Insecticides,World Health Organization. Expert committee on insecticides:seventh report[of a meeting held in Geneva from 10 to 17 July 1956] [R]. Geneva:WHO, 1957:1-31.
[3] Heckel DG. Genomics in pure and applied entomology[J]. Annu Rev Entomol, 2003, 48:235-260.
[4] Witzig C,Wondji CS,Strode C,et al. Identifying permethrin resistance loci in malaria vectors by genetic mapping[J]. Parasitology, 2013, 140(12):1468-1477.
[5] Irving H,Riveron JM,Ibrahim SS,et al. Positional cloning of rp2 QTL associates the P450 genes CYP6Z1,CYP6Z3 and CYP6M7 with pyrethroid resistance in the malaria vector Anopheles funestus[J]. Heredity (Edinb),2012,109(6):383-392.
[6] Neafsey DE, Waterhouse RM, Abai MR, et al. Mosquito genomics. Highly evolvable malaria vectors:the genomes of 16 Anopheles mosquitoes[J]. Science, 2015, 347(6217):1258522.
[7] Marinotti O, Cerqueira GC,De Almeida LGP,et al. The genome of Anopheles darlingi,the main neotropical malaria vector[J]. Nucleic Acids Res, 2013, 41(15):7387-7400.
[8] Zhou D, Liu XM, Sun Y, et al. Genomic analysis of detoxification supergene families in the mosquito Anopheles sinensis[J]. PLoS One, 2015, 10(11):e0143387.
[9] Adams MD,Celniker SE,Holt RA,et al. The genome sequence of Drosophila melanogaster[J]. Science,2000,287(5461):2185-2195.
[10] Holt RA,Subramanian GM,Halpern A,et al. The genome sequence of the malaria mosquito Anopheles gambiae[J]. Science, 2002, 298(5591):129-149.
[11] Xia QY,Zhou ZY,Lu C,et al. A draft sequence for the genome of the domesticated silkworm (Bombyx mori)[J]. Science, 2004, 306(5703):1937-1940.
[12] Nene V,Wortman JR,Lawson D,et al. Genome sequence of Aedes aegypti,a major arbovirus vector[J]. Science, 2007, 316(5832):1718-1723.
[13] You MS,Yue Z,He WY,et al. A heterozygous moth genome provides insights into herbivory and detoxification[J]. Nature Genetics, 2013, 45(2):220-225.
[14] Consortium IAG. Genome sequence of the pea aphid Acyrthosiphon pisum[J]. PLoS Biol, 2010, 8(2):e1000313.
[15] Dong ZC,Chen Y. Transcriptomics:advances and approaches[J]. Sci China Life Sci, 2013, 56(10):960-967.
[16] Mantione KJ,Kream RM,Kuzelova H, et al. Comparing bioinformatic gene expression profiling methods:microarray and RNA-Seq[J]. Med Sci Monit Basic Res, 2014, 20:138-141.
[17] Velculescu VE,Zhang L,Vogelstein B,et al. Serial analysis of gene expression[J]. Science, 1995, 270(5235):484-487.
[18] Brenner S,Johnson M,Bridgham J,et al. Gene expression analysis by massively parallel signature sequencing(MPSS)on microbead arrays[J]. Nat Biotechnol, 2000, 18(6):630-634.
[19] Sagri E, Reczko M, Gregoriou ME, et al. Olive fly transcriptomics analysis implicates energy metabolism genes in spinosad resistance[J]. BMC Genomics, 2014, 15:714.
[20] Bonizzoni M,Ochomo E,Dunn WA,et al. RNA-seq analyses of changes in the Anopheles gambiae transcriptome associated with resistance to pyrethroids in Kenya:identification of candidate-resistance genes and candidate-resistance SNPs[J]. Parasit Vectors, 2015, 8:474.
[21] Jones CM,Haji KA,Khatib BO,et al. The dynamics of pyrethroid resistance in Anopheles arabiensis from Zanzibar and an assessment of the underlying genetic basis[J]. Parasit Vectors, 2013, 6:343.
[22] Edi CV,Djogbénou L,Jenkins AM,et al. CYP6 P450 enzymes and ACE-1 duplication produce extreme and multiple insecticide resistance in the malaria mosquito Anopheles gambiae[J]. PLoS Genet, 2014, 10(3):e1004236.
[23] Mitchell SN,Stevenson BJ,Müller P,et al. Identification and validation of a gene causing cross-resistance between insecticide classes in Anopheles gambiae from Ghana[J]. Proc Natl Acad Sci USA, 2012, 109(16):6147-6152.
[24] Zhu GD,Zhong DB,Cao J,et al. Transcriptome profiling of pyrethroid resistant and susceptible mosquitoes in the malaria vector,Anopheles sinensis[J]. BMC Genomics, 2014, 15(1):448.
[25] Khajuria C,Buschman LL,Chen MS,et al. Identification of a novel aminopeptidase P-like gene(OnAPP)possibly involved in Bt toxicity and resistance in a major corn pest (Ostrinia nubilalis)[J]. PLoS One, 2011, 6(8):e23983.
[26] Karatolos N,Williamson MS,Denholm I,et al. Over-expression of a cytochrome P450 is associated with resistance to pyriproxyfen in the greenhouse whitefly Trialeurodes vaporariorum[J]. PLoS One, 2012, 7(2):e31077.
[27] Swinbanks D. Government backs proteome proposal[J]. Nature, 1995, 378(6558):653.
[28] Rappsilber J,Mann M. What does it mean to identify a protein in proteomics?[J]. Trends Biochem Sci, 2002, 27(2):74-78.
[29] Blackstock WP, Weir MP. Proteomics:quantitative and physical mapping of cellular proteins[J]. Trends Biotechnol, 1999, 17(3):121-127.
[30] Van Wijk KJ. Challenges and prospects of plant proteomics[J]. Plant Physiol, 2001, 126(2):501-508.
[31] Chevallet M,Santoni V,Poinas A,et al. New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis[J]. Electrophoresis, 1998, 19(11):1901-1909.
[32] Roepstorff P. Mass spectrometry in protein studies from genome to function[J]. Curr Opin Biotechnol, 1997, 8(1):6-13.
[33] Griffin TJ,Aebersold R. Advances in proteome analysis by mass spectrometry[J]. J Biol Chem, 2001, 276(49):45497-45500.
[34] Mann M,Hendrickson RC,Pandey A. Analysis of proteins and proteomes by mass spectrometry[J]. Annu Rev Biochem, 2001, 70:437-473.
[35] Pandey A,Mann M. Proteomics to study genes and genomes[J]. Nature, 2000, 405(6788):837-846.
[36] Yan DK,Hu M,Tang YX,et al. Proteomic analysis reveals resistance mechanism against chlorpyrifos in Frankliniella occidentalis (Thysanoptera:Thripidae)[J]. J Econ Entomol, 2015, 108(4):2000-2008.
[37] Wang WJ,Lv Y,Fang FJ,et al. Identification of proteins associated with pyrethroid resistance by iTRAQ-based quantitative proteomic analysis in Culex pipiens pallens[J]. Parasit Vectors, 2015, 8:95.
[38] Hollywood K,Brison DR,Goodacre R. Metabolomics:current technologies and future trends[J]. Proteomics, 2006, 6(17):4716-4723.
[39] Butcher P. A special section of reviews from BμG@S 2002:1^st symposium of the wellcome trust funded multi-collaborative microbial pathogen microarray facility[J]. Comp Funct Genomics, 2002, 3(4):325.
[40] Fiehn O. Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks[J]. Comp Funct Genomics, 2001, 2(3):155-168.
[41] Shulaev V. Metabolomics technology and bioinformatics[J]. Brief Bioinform, 2006, 7(2):128-139.
[42] Fiehn O,Kopka J,Trethewey RN,et al. Identification of uncommon plant metabolites based on calculation of elemental compositions using gas chromatography and quadrupole mass spectrometry[J]. Anal Chem, 2000, 72(15):3573-3580.
[43] Allen J,Davey HM,Broadhurst D,et al. High-throughput classification of yeast mutants for functional genomics using metabolic footprinting[J]. Nat Biotechnol,2003,21(6):692-696.
[44] Viant MR,Rosenblum ES, Tjeerdema RS. NMR-based metabolomics:a powerful approach for characterizing the effects of environmental stressors on organism health[J]. Environ Sci Technol, 2003, 37(21):4982-4989.
[45] Dettmer K,Aronov PA,Hammock BD. Mass spectrometry-based metabolomics[J]. Mass Spectrom Rev,2007,26(1):51-78.
[46] Kell DB. Metabolomics and systems biology:making sense of the soup[J]. Curr Opin Microbiol, 2004, 7(3):296-307.
[47] Stevenson BJ,Bibby J,Pignatelli P,et al. Cytochrome P450 6M2 from the malaria vector Anopheles gambiae metabolizes pyrethroids:sequential metabolism of deltamethrin revealed[J]. Insect Biochem Mol Biol, 2011, 41(7):492-502.
[48] Brinzer RA,Henderson L,Marchiondo AA,et al. Metabolomic profiling of permethrin-treated Drosophila melanogaster identifies a role for tryptophan catabolism in insecticide survival[J]. Insect Biochem Mol Biol, 2015, 67:74-86.