中国媒介生物学及控制杂志 ›› 2018, Vol. 29 ›› Issue (1): 61-63.DOI: 10.11853/j.issn.1003.8280.2018.01.015

• 论著 • 上一篇    下一篇

西藏自治区鼠疫自然疫源地鼠疫耶尔森菌耐药及耐消毒剂基因的研究

何建, 杨晓艳, 李胜, 靳娟, 张琪, 辛有全, 金泳, 熊浩明, 杨汉青, 魏柏青, 代瑞霞, 祁芝珍   

  1. 青海省地方病预防控制所鼠疫预防控制科, 西宁 811602
  • 收稿日期:2017-08-27 出版日期:2018-02-20 发布日期:2018-02-20
  • 通讯作者: 代瑞霞,Email:drx200907@163.com;祁芝珍,Email:qzz7777@163.com
  • 作者简介:何建,男,主管医师,从事鼠疫病原学研究,Email:550199930@qq.com
  • 基金资助:
    青海省自然科学基金(2016-ZJ-789);青海省鼠疫防控及研究重点实验室项目(2017-ZJ-Y22)

Study on the drug and disinfectant resistant genes of Yersina pestis in natural plague foci in Tibet

HE Jian, YANG Xiao-yan, LI Sheng, JIN Juan, ZHANG Qi, XIN You-quan, JIN Yong, XIONG Hao-ming, YANG Han-qing, WEI Bai-qing, DAI Rui-xia, QI Zhi-zhen   

  1. Qinghai Province Institute for Endemic Disease Control and Prevention, Xining 811602, Qinghai Province, China
  • Received:2017-08-27 Online:2018-02-20 Published:2018-02-20
  • Supported by:
    Supported by the Natural Science Foundation of Qinghai Province of China (No. 2016-ZJ-789) and Key Laboratory for Plague Prevention and Control of Qinghai Province (No. 2017-ZJ-Y22)

摘要: 目的 调查西藏自治区鼠疫自然疫源地是否存在耐药及耐消毒剂的鼠疫耶尔森菌(鼠疫菌)菌株,为鼠疫的临床治疗提供准确信息。方法 根据美国国立生物技术信息中心公布的耐氨基糖苷类链霉素StrAStrB基因,耐β-内酰胺类抗菌药物TEM、SHVCTX-M基因,耐磺胺类药物Sul1、Sul2Sul3基因序列,耐消毒剂耐药QacEdeltal-sul1基因,分别在每个基因上设计1对引物,逐一对分离自西藏自治区鼠疫自然疫源地的鼠疫菌DNA进行PCR检测。结果 阴性对照和阳性对照成立,355株鼠疫菌的PCR检测结果均为阴性,未发现耐链霉素、耐磺胺类药物及耐β-内酰胺类抗菌药物和耐消毒剂菌株。结论 西藏自治区鼠疫自然疫源地鼠疫菌尚未出现耐药及耐消毒剂菌株。

关键词: 鼠疫耶尔森菌, 西藏自治区, 耐药基因, 耐消毒剂基因

Abstract: Objective To understand whether there is a drug-or disinfectant-resistant strains in natural plague foci in Tibet, and provide the accurate information for clinical treatment of plague. Methods According to the aminoglycoside resistant gene of streptomycin resistant, StrB, StrA, beta lactam antibiotics TEM, SHV, and CTX-M gene, sulfamilamide resistant Sul1, Sul2, and Sul3, and anti-disinfectant QacEdeltal-sul1 gene sequence the National Center for Biotechnology Information (NCBI) released,a pair of primers in each gene was designed separately. DNA of strains isolated from natural plague foci in Tibet were amplified by PCR using every pair of primers. Results Negative and positive control were established, samples by PCR amplification results were negative, there were no streptomycin, sulfamilamide and beta lactam antimicrobial drug resistance genes and anti-disinfectant genes in strains studied. Conclusion The Tibet autonomous region of natural plague foci did not appear to have drug-or disinfectant-resistant Yersina pestis.

Key words: Yersina pestis, Tibet, Resistance genes, Disinfectant resistant genes

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