中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (6): 600-602.DOI: 10.11853/j.issn.1003.8280.2017.06.022

• 调查研究 • 上一篇    下一篇

采用3种钉螺孵化方法检测有螺土壤中活性螺卵的比较研究

田建国1, 黎桂福1, 彭丽霞2, 蒋守富3, 张小萍3, 李俊1   

  1. 1 上海市青浦区疾病预防控制中心综合业务科, 上海 201700;
    2 湖南省中医药研究院附属医院, 长沙 410006;
    3 上海市疾病预防控制中心, 上海 200336
  • 收稿日期:2017-08-15 出版日期:2017-12-20 发布日期:2017-12-20
  • 作者简介:田建国,男,主任医师,从事传染病防制及相关病媒生物控制,Email:jgtian@126.com
  • 基金资助:
    上海市青浦区科学技术发展基金(青科发2015-33)

A comparative study on hatching active eggs of Oncomelania hupensis in soil by three methods

TIAN Jian-guo1, LI Gui-fu1, PENG Li-xia2, JIANG Shou-fu3, ZHANG Xiao-ping3, LI Jun1   

  1. 1 Qingpu District Center for Disease Control and Prevention, Shanghai 201700, China;
    2 The Attached Hospital of Chinese Medicine Institute of Hunan Province;
    3 Shanghai Center for Disease Control and Prevention
  • Received:2017-08-15 Online:2017-12-20 Published:2017-12-20
  • Supported by:
    Supported by the Science and Technology Development Fund from Qingpu District Shanghai Municipal(No. 2015-33)

摘要: 目的 通过对有螺地区土壤样本进行检测,比较3种孵化螺卵检测方法,探讨现场适用的简易检测方法。方法 于2016年5月8日在上海市青浦区有螺地区采集土壤20块,柔性混匀后,每块土壤分别称取3份(每份500 g),采用收集螺卵恒温孵化法、直接孵化法和改良直接孵化法进行孵化实验,采用Kappa值进行一致性检验;检出幼螺的孵化时间采用Kruskal-Wallis检验;3种孵化方法孵化率的差异采用χ2检验。结果 收集螺卵恒温孵化法和改良直接孵化法温度差异有统计学意义(t=7.309,P=0.000);3种方法孵化出幼螺时间经Kruskal-Wallis检验差异有统计学意义(χ2=269.340,P < 0.001),孵化率差异无统计学意义(χ2=5.880,P=0.053)。结论 3种方法均可在有螺地区土壤中孵化出钉螺,但直接孵化法实验条件简单、易于操作,可作为土壤中检测钉螺螺卵的补充方法,并应用于现场实验。

关键词: 收集螺卵恒温孵化法, 直接孵化法, 改良直接孵化法, 钉螺, 螺卵

Abstract: Objective To explore an easy and feasible method for hatching snail eggs in soil where snails existed. Methods On May 8th, 2016, 20 soil samples were taken from Qingpu district where snails existed, and 3 units of soil which weighed 500 g respectively were used to carry on the experiment to hatch snail eggs in each piece of soil. The three methods included hatching snail eggs directly in the natural conditions, hatching eggs with improved conditions and hatching eggs with stable temperature. Kappa test was used to analyze the consistence of the results of the three methods. Kruskal-Wallis test was used to compare the hatching time, and χ2 test was used to compare the three rates of hatching eggs. Results There was a statistical significance between methods with stable temperature and improved conditions in temperature (t=7.309, P=0.000). There were statistical significances in periods of hatching snail eggs among the three methods by Kruskal-Wallis test (χ2=269.340, P<0.001). There was no statistical significance in hatching rates with the three methods (χ2=5.880, P=0.053). Conclusion All the three methods can hatch snail eggs in the soil where snails existed, while the method hatching snail eggs directly under natural conditions canbe used as a complementary method since it needed simple experiment conditions.

Key words: Hatching with stable temperature, Hatching directly in natural conditions, Hatching with improved conditions, Oncomelania hupensis, Snail eggs

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