中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (3): 233-237.DOI: 10.11853/j.issn.1003.8280.2017.03.009

• 论著 • 上一篇    下一篇

新疆地区58株鼠疫耶尔森菌规律聚集的间隔短回文重复位点多态性分析

贺金荣1, 张渝疆2, 王宇萌1, 蔡虹1, 张志凯1, 李伟1, 梁莹1   

  1. 1 中国疾病预防控制中心传染病预防控制所鼠疫室, 北京 102206;
    2 新疆维吾尔自治区疾病预防控制中心, 乌鲁木齐 830001
  • 收稿日期:2017-01-16 出版日期:2017-06-20 发布日期:2017-06-20
  • 通讯作者: 梁莹,Email:liangying@icdc.cn
  • 作者简介:贺金荣,女,主管技师,主要从事鼠疫实验室检测技术研究,Email:hejinrong@icdc.cn;张渝疆,男,主任医师,主要从事鼠疫防治及病原学研究,Email:xjsyzhang@163.com
  • 基金资助:
    国家重大科学仪器设备开发专项(2012YQ09019706)

Polymorphism analysis of clustered regularly interspaced short palindromic repeat (CRISPR) loci of 58 Yersinia pestis strains isolated from Xinjiang, China

HE Jin-rong1, ZHANG Yu-jiang2, WANG Yu-meng1, CAI Hong1, ZHANG Zhi-kai1, LI Wei1, LIANG Ying1   

  1. 1 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
    2 Xinjiang Center for Disease Control and Prevention
  • Received:2017-01-16 Online:2017-06-20 Published:2017-06-20
  • Supported by:
    Supported by the National Priority Development Project on Key Science Instrument(No. 2012YQ09019706)

摘要: 目的 了解新疆维吾尔自治区(新疆)鼠疫耶尔森菌(鼠疫菌)规律聚集的间隔短回文重复(CRISPR)位点的遗传多态性,对菌株进行基因分型。方法 利用PCR技术对分离自新疆的58株鼠疫菌的基因组DNA进行扩增,测定扩增产物的核酸序列,分析各菌株CRISPR位点的间区序列种类和排列情况,应用Bionumerics软件制作聚类图,确定菌株基因型。结果 共鉴定42个间区序列和23种间区序列阵列,58株鼠疫菌分为16个基因型,即基因3型、8型、11型和16型,主要分布在新疆乌恰县、和田地区、尼勒克和玛纳斯县;基因7型的鼠疫菌全部分离自新疆准噶尔盆地大沙鼠及其体表寄生蚤;其他型别的鼠疫菌则主要分布在新疆北天山和准噶尔盆地。结论 新疆地区的鼠疫菌CRISPR位点遗传多态性较高,不同基因型的鼠疫菌地理分布有明显的区域性特征。

关键词: 鼠疫耶尔森菌, 多态性, 新疆维吾尔自治区

Abstract: Objective To study the genetic polymorphism of clustered regularly interspaced short palindromic repeat (CRISPR)loci in the Yersinia pestis strains from Xinjiang Uygur Autonomous Region, China and then to identify the genotypes of tested strains. Methods Genomic DNA of 58 Y. pestis strains from Xinjiang was amplified using PCR technique, and the nucleic acid sequences of PCR production were then determined. On each CRISPR locus of every strain, spacers and spacer arrays were analyzed according to the PCR production sequences. Genotypes of tested strains were identified through the dendrogram generated by software. Results A total of 42 spacers and 23 spacer arrays were confirmed and 58 Y. pestis strains were divided into 16 genotypes. Yersinia pestis strains of genotype 3, 8, 11 and 16 distributed intensively in Wuqia county, Hotan prefecture, Nilka county and Manasi county respectively; strains of genotype 7 were all isolated from Rhombomys opimus and parasitic fleas in vitro in Junggar Basin; strains of the other genotypes mainly distributed in North Tianshan and Junggar Basin. Conclusion The genetic polymorphism of CRISPR loci in Xinjiang Y. pestis strains is high, and the geographical distribution of Y. pestis strains of different genotypes has obviously regional characteristics.

Key words: Yersinia pestis, Polymorphism, Xinjiang Uygur Autonomous Region

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