基于DNA条形码和子宫骨片形态特征的8种雌性麻蝇种类鉴定

doi:10.11853/j.issn.1003.8280.2016.05.004

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (5): 436-442.DOI: 10.11853/j.issn.1003.8280.2016.05.004

基于DNA条形码和子宫骨片形态特征的8种雌性麻蝇种类鉴定

岳巧云^1,2, 邱德义², 单振菊², 汪小东², 胡佳², 张文庆¹

1 中山大学有害生物控制与资源利用国家重点实验室, 广东广州 510275;
2 中山出入境检验检疫局检验检疫技术中心, 广东中山 528403

收稿日期:2016-06-16 出版日期:2016-10-20 发布日期:2016-10-20
通讯作者: 邱德义,Email:qiudy@zs.gdciq.gov.cn
作者简介:岳巧云,女,副研究员,从事媒介生物形态和分子鉴定工作,Email:yueqy@zs.gdciq.gov.cn
基金资助:
国家科技支撑计划课题（2012BAK11B05）；国家质检总局项目（2015IK067，2015IK069）；广东省科技项目（2015A050502009）

Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics

YUE Qiao-yun^1,2, QIU De-yi², SHAN Zhen-ju², WANG Xiao-dong², HU Jia², ZHANG Wen-qing¹

1 State Key Laboratory of Pest Control and Resources Utilization, Sun Yat-Sen University, Guangzhou 510275, Guangdong Province, China;
2 Zhongshan Entry-Exit Inspection and Quarantine Technology Center

Received:2016-06-16 Online:2016-10-20 Published:2016-10-20
Supported by:
Supported by the National Science and Technology Infrastructure Program (No. 2012BAK11B05), Administration of Quality Supervision, Inspection and Quarantine Support Program of China (No. 2015IK067, 2015IK069), and Science and Technology Project of Guangdong Province (No. 2015A050502009)

摘要/Abstract

摘要：

目的建立一种准确鉴定雌性麻蝇物种的可靠方法。方法 2011年10月25日至2013年10月27日，共收集35对正在交配的8种麻蝇及57只雄性个体并获取其DNA条形码序列，依据雄性麻蝇的尾器特征进行种类鉴定；从GenBank下载11条该8种麻蝇序列，比较种内所有个体、雌雄个体及不同种类间DNA条形码序列的差异度。雌性个体的种类根据与其交配的雄性个体，并结合DNA条形码确定，详细描述8种雌性麻蝇子宫骨片的主要形态特征，并依据其物证编写种类鉴定检索表。结果 8种麻蝇的同种雌雄个体间DNA条形码序列差异范围为0～1.37%，与同种个体间的差异范围相符，且<2%的DNA条形码种类鉴定的界限，不同种类间DNA条形码差异范围为4.56%～8.81%，与种内不同个体间有明显差异。不同种类雌性麻蝇的子宫骨片结构具有种的特异性，可作为雌性麻蝇种类鉴定的依据。结论 DNA条形码可准确地鉴定雌性个体，并进一步发展雌性的某种独特的形态特性，为实现雌性个体的直接形态鉴定提供依据。

关键词: 雌性麻蝇, DNA条形码, 子宫骨片形态特征

Abstract:

Objective To establish a method to accurately identify the species of the female Sarcopha. Methods Thirty five mating pairs of eight Sarcopha species and 57 single male individuals of these 8 species were collected from October 25 in 2011 to October 27 in 2013. Their DNA barcodes were sequenced and 11 DNA barcodes sequences of these 8 species were also downloaded from the GenBank. Males were identified according to their genitalia, DNA barcodes differences between the intra-species, inter-sexes and inter-species were compared. Females were identified according to their mating males and the DNA barcodes, female's signum morphological characteristics of these eight species were illustrated and a key based on it was constructed. Results Inter-sexes DNA barcodes differences were 0-1.37% of these 8 species, and were comparable to the intra-species variations, and were lower than the 2% species limitation. The inter-species DNA barcodes differences were 4.56%-8.81%, there are distinct gaps between the intra-species and inter-species. The signum morphological characteristics were species-specific and could be used to identify the females. Conclusion DNA barcoding is a practical tool to identify the female Sarcopha. Species-specific morphological characters of the female could be developed based on the identification result of DNA barcoding, then provides references for the direct morphological identification of the females.

Key words: Female Sarcopha flies, DNA barcoding, Signum morphological characteristics

中图分类号:

R384.2

岳巧云, 邱德义, 单振菊, 汪小东, 胡佳, 张文庆. 基于DNA条形码和子宫骨片形态特征的8种雌性麻蝇种类鉴定[J]. 中国媒介生物学及控制杂志, 2016, 27(5): 436-442.

YUE Qiao-yun, QIU De-yi, SHAN Zhen-ju, WANG Xiao-dong, HU Jia, ZHANG Wen-qing. Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics[J]. Chines Journal of Vector Biology and Control, 2016, 27(5): 436-442.

参考文献

[1] Greenberg B. Flies and disease: vol. 1,ecology, classification and biotic associations[M]. New Jersey,USA:Princeton University Press,1971:1-5.
[2] Sukontason KL,Bunchu N,Methanitikorn R,et al. Ultrastructure of adhesive device in fly in the families Calliphoridae, Muscidae and Sarcophagidae, and their implication as mechanical carriers of pathogens[J]. Parasitol Res,2006,98(5):477-481.
[3] Pape T. The Sarcophagidae (Diptera) of fennoscandia and denmark: fauna entomologica scandinavica[M]. Leiden, Holland:Dutch Academic Publishing House Brill,1987:1-203.
[4] Pape TH. Catalogue of the Sarcophagidae of the world (Insecta:Diptera): memoirs on entomology, international, Volume: 8[M]. Gainesville,Florida,USA:American Entomological Institute,1996:3-7.
[5] 范滋德. 中国常见蝇类检索表[M]. 2版. 北京:科学出版社, 1992:580-718.
[6] 薛万琦,赵建铭. 中国蝇类[M]. 沈阳:辽宁科学技术出版社, 1996:1518-1656.
[7] Byrd JH, Castner JL. Forensic entomology: the utility of arthropods in legal investigations[M]. 2nd ed. London, England: CRC Press Taylor & Francis Group,2010:1-10.
[8] Hebert PDN, Ratnasingham S, de Waard JR. Barcoding animal life: cytochrome c oxidase subunitⅠ divergences among closely related species[J]. Proc Roy Soc B Biol Sci,2003,270 Suppl 1: S96-99.
[9] Hebert PDN, Cywinska A, Ball SL. Biological identifications through DNA barcodes[J]. Proc Roy Soc B Biol Sci,2003,270(1512):313-321.
[10] Blaxter M, Mann J, Chapman T, et al. Defining operational taxonomic units using DNA barcode data[J]. Philos Trans Roy Soc B Biol Sci,2005,360(1462):1935-1943.
[11] Evans KM, Wortley AH, Mann DG. An assessment of potential diatom “barcode” genes (cox1, rbcL, 18S and ITS rDNA) and their effectiveness in determining relationships in Sellaphora (Bacillariophyta)[J]. Protist,2007,158(3):349-364.
[12] Hebert PDN, Penton EH, Burns JM, et al. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator[J]. Proc Natl Acad Sci USA,2004,101(41):14812-14817.
[13] Hajibabaei M, Singer GAC, Clare EL, et al. Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring[J]. BMC Biol,2007,5(1):24.
[14] Yang ZH, Rannala B. Bayesian species delimitation using multilocus sequence data[J]. Proc Natl Acad Sci USA,2010, 107(20):9264-9269.
[15] Bergsten J, Bilton DT, Fujisawa T, et al. The effect of geographical scale of sampling on DNA barcoding[J]. Syst Biol, 2012,61(5):851-869.
[16] Vernooy R, Haribabu E, Muller MR, et al. Barcoding life to conserve biological diversity: beyond the taxonomic imperative [J]. PLoS Biol,2010,8(7):e1000417.
[17] Tautz D, Arctander P, Minelli A, et al. A plea for DNA taxonomy[J]. Trends Ecol Evol,2003,18(2):70-74.
[18] Dayrat B. Towards integrative taxonomy[J]. Biol J Linn Soc, 2005,85(3):407-415.
[19] Padial JM, Miralles A, De la Riva I, et al. Review: the integrative future of taxonomy[J]. Front Zool,2010,7(1):16.
[20] Bergsten J, Brilmyer G, Crampton-Platt A, et al. Sympatry and colour variation disguised well-differentiated sister species: Suphrodytes revised with integrative taxonomy including 5 kbp of housekeeping genes (Coleoptera:Dytiscidae)[J]. DNA Barcodes,2012,1:1-18.
[21] Yue QY, Wu KL, Qiu DY, et al. A formal re-description of the cockroach Hebardina concinna anchored on DNA barcodes confirms wing polymorphism and identifies morphological characters for field identification[J]. PLoS One,2014,9(9): e106789.
[22] Folmer O, Black M, Hoeh W, et al. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol, 1994, 3(5): 294-299.
[23] Povolný D,Verves Y. The flesh-flies of central Europe (Insecta, Diptera, Sarcophagidae)[J]. Spixiana,1997,24:1-260.
[24] Renaud AK,Savage J,Adamowicz SJ. DNA barcoding of northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits[J]. BMC Ecol,2012,12:24.
[25] Rivera J, Currie DC. Identification of Nearctic black flies using DNA barcodes (Diptera:Simuliidae)[J]. Mol Ecol Resour, 2009,9 Suppl 1:S224-236.
[26] e Schuehli GS, de Carvalho CJB, Wiegmann BM. Molecular phylogenetics of the Muscidae (Diptera: Calyptratae): new ideas in a congruence context[J]. Invertebr Syst,2007,21(3): 263-278.
[27] 叶宗茂. 麻蝇族3种雌性麻蝇的发现(双翅目:麻蝇科)[J]. 动物分类学报,1985(1):107-110.
[28] Wells JD, Pape T, Sperling FA. DNA-based identification and molecular systematics of forensically important Sarcophagidae (Diptera)[J]. J Forensic Sci,2001,46(5):1098-1102.

基于DNA条形码和子宫骨片形态特征的8种雌性麻蝇种类鉴定

Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics

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