青藏高原喜马拉雅旱獭粪便携带腹泻相关病毒的调查分析

doi:10.11853/j.issn.1003.8280.2016.04.005

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (4): 333-335.DOI: 10.11853/j.issn.1003.8280.2016.04.005

青藏高原喜马拉雅旱獭粪便携带腹泻相关病毒的调查分析

罗雪莲^1,2, 郑雅匀¹, 戴晓懿¹, 金东¹, 孙晖¹, 熊衍文^1,2

1 中国疾病预防控制中心传染病预防控制所新病原室, 传染病预防控制国家重点实验室, 北京 102206;
2 感染性疾病诊治协同创新中心, 浙江杭州 310003

收稿日期:2016-03-02 出版日期:2016-08-20 发布日期:2016-08-20
通讯作者: 熊衍文,Email:xiongyanwen@icdc.cn
作者简介:罗雪莲,女,博士,副研究员,主要从事未知病原体的分离、鉴定等工作,Email:luoxuelian@icdc.cn
基金资助:
病毒学国家重点实验室开放课题（2015KF003）；国家科技重大专项（2013ZX10004-101）

Investigation and sequence analysis of diarrhea related viruses in the feces of Marmota himalayana on the Qinghai-Tibet Plateau, China

LUO Xue-lian^1,2, ZHENG Ya-yun¹, DAI Xiao-yi¹, JIN Dong¹, SUN Hui¹, XIONG Yan-wen^1,2

1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Received:2016-03-02 Online:2016-08-20 Published:2016-08-20
Supported by:
Supported by the Open Research Fund Program of the State Key Laboratory of Virology of China(No. 2015KF003)and the National Science and Technology Major Project of China(No. 2013ZX10004-101)

摘要/Abstract

摘要：

目的调查我国青藏高原喜马拉雅旱獭粪便中腹泻相关病毒的携带情况，并对检出病毒进行序列分析。方法根据腹泻相关病毒基因组保守区设计兼并引物，采用直接PCR或一步法RT-PCR方法对96份喜马拉雅旱獭粪便标本核酸进行检测；阳性PCR产物测序后与参考毒株进行序列比对；利用PhyML 3.0软件对喜马拉雅旱獭粪便中病毒序列进行进化分析。结果 96份青藏高原喜马拉雅旱獭粪便中共检出星状病毒32份、小双节RNA病毒42份和嵴病毒5份，阳性率分别为35.4%、43.8%和5.2%；星状病毒、小双节RNA病毒和嵴病毒与参考毒株核苷酸一致性分别为78%、77%和93%，氨基酸一致性分别为77%、84%和91%。兼并引物未检测到腺病毒、札如病毒和诺如病毒。结论青藏高原喜马拉雅旱獭粪便中存在星状病毒、小双节RNA病毒和嵴病毒，为后续喜马拉雅旱獭携带病毒谱系研究提供了重要线索。

关键词: 喜马拉雅旱獭, 星状病毒, 小双节RNA病毒, 嵴病毒

Abstract:

Objective To investigate the diarrhea associated viruses in the feces of Marmota himalayana on the Qinghai- Tibet Plateau, China, and also analyze these viruses sequences. Methods We designed the primers according the conserved region of genome, and detected 96 fecal samples by direct PCR or one-step RT-PCR; the positive PCR products were sequenced and aligned with the reference strains using PhyML 3.0 software. We also analyzed the evolution of these virus's sequences. Results A total of 32 astroviruses, 42 picobirnaviruses, and 5 kobuviruses were detected in 96 feces of M. himalayana. The positive of these viruses were 35.4%, 43.8%, and 5.2%, respectively. These virus's sequences had a nucleotide homology of 78%, 77%, and 93% and the amino acid homology of 77%, 84%, and 91% with the reference strains, respectively. Adenovirus, sapovirus and norovirus were not detected by the degenerate primers. Conclusion There are picobirnaviruses, astroviruses and kobuviruses in the feces of M. himalayana. These studies will provide important clues for further study of viruses in M. himalayana.

Key words: Marmota himalayana, Astrovirus, Picobirnavirus, Kobuvirus

中图分类号:

R373
S852.65

罗雪莲, 郑雅匀, 戴晓懿, 金东, 孙晖, 熊衍文. 青藏高原喜马拉雅旱獭粪便携带腹泻相关病毒的调查分析[J]. 中国媒介生物学及控制杂志, 2016, 27(4): 333-335.

LUO Xue-lian, ZHENG Ya-yun, DAI Xiao-yi, JIN Dong, SUN Hui, XIONG Yan-wen. Investigation and sequence analysis of diarrhea related viruses in the feces of Marmota himalayana on the Qinghai-Tibet Plateau, China[J]. Chines Journal of Vector Biology and Control, 2016, 27(4): 333-335.

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青藏高原喜马拉雅旱獭粪便携带腹泻相关病毒的调查分析

Investigation and sequence analysis of diarrhea related viruses in the feces of Marmota himalayana on the Qinghai-Tibet Plateau, China

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