中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (4): 409-410.DOI: 10.11853/j.issn.1003.4692.2015.04.021

• 调查研究 • 上一篇    下一篇

登封市一株小肠结肠炎耶尔森菌带毒株分析

张胜勇1, 梅志敏2, 李洪民1, 张锦3, 王德祥1, 夏胜利3   

  1. 1 登封市卫生防疫站, 河南登封452470;
    2 登封市人民医院;
    3 河南省疾病预防控制中心, 郑州450016
  • 收稿日期:2015-02-23 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 夏胜利,Email: xiasl@hncdc.com.cn
  • 作者简介:张胜勇,男,硕士,主管检验师,从事微生物学检验研究,Email: zsy0371@163.com
  • 基金资助:

    国家科技重大专项课题(2013ZX10004203-002-002)

Analysis on a pathogenic strain of Yersinia enterocolitica in Dengfeng city, Henan, China

ZHANG Sheng-yong1, MEI Zhi-min2, LI Hong-min1, ZHANG Jin3, WANG De-xiang1, XIA Sheng-li3   

  1. 1 Dengfeng Hygiene and Anti-Epidemic Station, Dengfeng 452470, Henan Province, China;
    2 The People's Hospital of Dengfeng City;
    3 Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan Province, China
  • Received:2015-02-23 Online:2015-08-20 Published:2015-08-20
  • Supported by:

    Supported by the Major National Science and Technology Projects of China(No. 2013ZX10004203-002-002)

摘要:

目的 了解登封市1株引起幼儿腹泻的小肠结肠炎耶尔森菌的血清型、生物型、毒力基因携带情况。方法 通过细菌培养、血清凝集法及PCR检测技术对分离菌株进行血清分型、毒力基因检测。结果 从幼儿采集的粪便标本中检出小肠结肠炎耶尔森菌,镜下见革兰染色阴性短杆菌。该菌氧化酶阴性,触酶阳性,无硫化氢产生,尿素酶试验阳性,ONPG(邻硝基酚-半乳糖苷酶)试验阳性,血清分型为O:3,生物型为3型。该菌株O:3血清型阳性,生物型3型,毒力基因ail+ystA+ystB-yadA+virF+结论 引起幼儿腹泻的病原菌为致病性小肠结肠炎耶尔森菌。

关键词: 腹泻病例, 带毒株, 小肠结肠炎耶尔森菌

Abstract:

Objective To understand the strain of serotype, biotype, virulence genes, which cause infantile diarrhea. Methods Bacterial culture, serum agglutination method and PCR were used for serotyping, detection of virulence genes. Results A strain of Yersinia enterocolitica was isolated from the children of feces sample and the short bacillus of gram stain negative was identified under the microscope. Oxidase of the bacteria was negative, katalase was positive, no hydrogen sulfide production, urease test positive, ONPG test positive. The Y. enterocolitica strain is positive in O:3 serotyping test and it belongs to 3 biological types, carrying the virulence genes of ail+, ystA+, yadA+, virF+ but no ystB- . Conclusion The case of infantile diarrhea disease is caused by pathogenic Y. enterocolitica.

Key words: Diarrhea disease, Toxicity-carrying strain, Yersinia enterocolitica

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