中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (2): 141-144.DOI: 10.11853/j.issn.1003.4692.2015.02.008

• 论著 • 上一篇    下一篇

河北省鼠疫耶尔森菌多位点串联重复序列分析

王海峰1, 杨顺林1, 周松1, 史献明1, 胡乐乐1, 刘合智1, 梁莹2   

  1. 1 河北省鼠疫防治所检验科, 河北张家口075000;
    2 中国疾病预防控制中心传染病预防控制所
  • 收稿日期:2014-10-13 出版日期:2015-04-20 发布日期:2015-04-20
  • 通讯作者: 史献明,Email:wolfshi9974@sina.com
  • 作者简介:王海峰,女,硕士,微生物检验师,从事鼠疫检验研究,Email:whf584521@163.com
  • 基金资助:

    卫生部行业基金(201202021);河北省2014年度医学科学研究重点课题计划(ZL20140119)

MLVA analysis on Yersinia pestis isolated from plague foci in Hebei province, China

WANG Hai-feng1, YANG Shun-lin1, ZHOU Song1, SHI Xian-ming1, HU Le-le1, LIU He-zhi1, LIANG Ying2   

  1. 1 Anti-plague Institute of Hebei Province, Zhangjiakou 075000, Hebei Province, China;
    2 National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:2014-10-13 Online:2015-04-20 Published:2015-04-20
  • Supported by:

    Supported by the Special Research Program for Health(No. 201202021)and the Medical Science Research Key Project in Hebei Province(No. ZL20140119)

摘要:

目的 对分离自河北省长爪沙鼠鼠疫疫源地的116株鼠疫菌株进行基因分析研究。方法 根据鼠疫基因组比对, 选择15对引物对测试菌株进行PCR扩增, 并对扩增结果进行分析。结果 15对引物对116株实验菌株均得到相应的扩增结果, 同一引物测试菌株扩增结果目标条带长度均一致, 串联重复序列的重复数相同。不同引物扩增目标条带长度不同, 串联重复序列的重复数也不同, 如引物M52对所有菌株扩增产物长度均为153 bp, 串联重复序列的重复数均为3, 而引物M59的扩增产物长度为250 bp, 重复数均为7。结论 多位点串联重复序列分析(MLVA)证实河北省鼠疫自然疫源地的鼠疫菌基因稳定, 鼠疫菌MLVA数据库将对未来的鼠疫菌变异和溯源提供技术支持。

关键词: 鼠疫菌, 多位点串联重复序列分析, 基因分型

Abstract:

Objective To characterize the genosome of 116 Yersinia pestis strains isolated from plague foci in Hebei province in China. Methods All the strains were detected by polymerase chain reaction (PCR) with 15 pairs variable number tandem repeat (VNTR) provided by China CDC, then the length of results were analyzed. Results The numbers and lengths of the repeat sequences from all strains are same with the single primer, and different with the different primers, such as the length of all stains with the prime M52 is 153 bp, and the number of the repeat sequences is 3, then with the prime M59, the length and the number is 250 bp and 3 respectively. Conclusion Multiple loci VNTR analysis (MLVA) genetic typing is reliable, and it is stable genetic mark of Y. pestis from Hebei province. To build the database of the plague with MLVA is useful for the investigation of the plague variation and source.

Key words: Yersinia pestis, Multiple loci VNTR analysis, Genetic typing

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