中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (5): 432-434.DOI: 10.11853/j.issn.1003.4692.2014.05.012

• 论著 • 上一篇    下一篇

用DNA条形码技术鉴定阿拉善黄鼠和达乌尔黄鼠

陈宝宝, 孙养信, 安翠红, 霍丽霞, 范锁平   

  1. 陕西省疾病预防控制中心鼠布生防科,西安 710054
  • 收稿日期:2014-04-25 出版日期:2014-10-20 发布日期:2014-10-20
  • 通讯作者: 孙养信,Email: sxpco@126.com
  • 作者简介:陈宝宝,女,硕士,微生物检验技师,主要从事鼠疫、布鲁氏菌病及病媒生物实验研究,Email: cb92118019@163.com
  • 基金资助:
    陕西省科学技术研究发展计划项目(2012K16-12-03)

Identification of Spermophilus alaschanicus and Spermophilus dauricusby DNA barcoding

CHEN Bao-bao, SUN Yang-xin, AN Cui-hong, HUO Li-xia, FAN Suo-ping   

  1. Shaanxi Center for Disease Control and Prevention, Xi’an 710054, Shaanxi Province, China
  • Received:2014-04-25 Online:2014-10-20 Published:2014-10-20
  • Contact: SUN Yang-xin, Email: sxpco@126.com
  • Supported by:
    Supported by the Science and Technology Research and Development Program of Shaanxi Province (No. 2012K16-12-03)

摘要: 目的 探索阿拉善黄鼠和达乌尔黄鼠的分类鉴定方法。方法 运用DNA条形码技术测定两地黄鼠的线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列,分析遗传距离,构建系统发育NJ树。结果 11份阿拉善黄鼠的种内遗传距离<2%,4份达乌尔黄鼠的种内遗传距离<1%,两地黄鼠的遗传距离介于8%~9%之间,同时,NJ树将两地黄鼠分成2个独立分支。结论 阿拉善黄鼠为独立物种。DNA条形码技术可用于黄鼠种类鉴定。

关键词: DNA条形码, 细胞色素C氧化酶亚基Ⅰ基因, 鼠种鉴定, 阿拉善黄鼠, 达乌尔黄鼠

Abstract: Objective To investigate the classification and identification methods for Spermophilus alaschanicus and S. dauricus. Methods DNA barcoding was used to determine the cytochrome c oxidase subunitⅠ (COⅠ) gene sequences of S. alaschanicus and S. dauricus, analyze the genetic distance, and construct a neighbor-joining (NJ) tree. Results  The intraspecific genetic distance of S. alaschanicus (n=11) was less than 2%, and that of S. dauricus (n=4) was less than 1%. The genetic distance between S. alaschanicus and S. dauricus ranged from 8% to 9%. Results of NJ tree showed that all the samples of the two species formed two independent groups with a high support value. Conclusion Spermophilus alaschanicus is an independent species. DNA barcoding can be used in the species identification of Spermophilus.

Key words: DNA barcoding, COⅠ gene, Identification, Spermophilus alaschanicus, Spermophilus dauricus

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