淡色库蚊抗药性测定中不同容器对测定结果的影响研究

doi:10.11853/j.issn.1003.4692.2014.05.001

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (5): 385-387.DOI: 10.11853/j.issn.1003.4692.2014.05.001

• 论著 • 下一篇

淡色库蚊抗药性测定中不同容器对测定结果的影响研究

林国松^{1, 2}, 张晓越¹, 刘欣¹, 孟凤霞¹

1 中国疾病预防控制中心传染病预防控制所媒介生物控制室,传染病预防控制国家重点实验室,世界卫生组织媒介生物监测与管理合作中心,北京 102206;
2 泰山医学院公共卫生学院,山东泰安 271016

收稿日期:2014-04-30 出版日期:2014-10-20 发布日期:2014-10-20
通讯作者: 孟凤霞,Email: mengfengxia@icdc.cn
作者简介:林国松,男,主要从事传染病媒介蚊虫的控制技术研究,Email: linguosong90@163.com
基金资助:
国家科技重大专项课题(2012ZX10004-219)

Influence of containers on insecticide resistance bioassayin Culex pipiens pallens larvae

LIN Guo-song^1,2, ZHANG Xiao-yue¹, LIU Xin¹, MENG Feng-xia¹

1 WHO Collaborating Centre for Vector Surveillance and Management, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 College of Public Health,Taishan Medical University, Tai’an 271016, Shandong Province

Received:2014-04-30 Online:2014-10-20 Published:2014-10-20
Contact: MENG Feng-xia, Email: mengfengxia@icdc.cn
Supported by:
Supported by the National Science and Technology Major Projects of China (No. 2012ZX10004-219)

摘要/Abstract

摘要： 目的　评估淡色库蚊抗药性测定中容器类型对结果的影响,确定能否用一次性杯子代替玻璃烧杯进行实验,从而达到提高实验效率的目的。方法　从野外采集淡色库蚊幼虫,用WHO推荐的幼虫浸渍法,分别测定在玻璃烧杯、一次性塑料杯或一次性纸杯3种容器类型下,淡色库蚊幼虫对溴氰菊酯、仲丁威和双硫磷的LC50及95%可信区间(95%CI),并比较其差异。结果　在玻璃烧杯、一次性塑料杯和一次性纸杯依次作为实验容器的条件下,淡色库蚊幼虫对溴氰菊酯的LC50及95%CI分别为0.002 009(0.001 654～0.002 449)、0.003 858(0.003 062～0.005 224)和0.005 459(0.004 051～0.008 757) mg/L,玻璃杯与2种一次性杯子间的结果差异有统计学意义;对仲丁威的LC50及95%CI分别为0.3470(0.3059～0.3821)、0.2575(0.2118～0.3095)和0.4844(0.4460～0.5232) mg/L,玻璃杯与一次性塑料杯差异无统计学意义,与一次性纸杯差异有统计学意义;而对双硫磷的LC50及95%CI分别为0.000 955(0.000 816～0.001 094)、0.003 007(0.002 661～0.003 407)和0.003 830(0.003 450～0.004 256) mg/L,玻璃杯与2种一次性杯子间差异均有统计学意义。用不同杯子测定蚊幼虫对杀虫剂的敏感性,溴氰菊酯的测定结果受浓度的影响小于双硫磷;双硫磷浓度越高杯子的影响越小。结论　在对蚊幼虫的抗药性测定中,玻璃杯是最佳选择,而一次性杯子可能因对杀虫剂有吸附作用而使测定的结果发生偏差,使用一次性杯子替代玻璃杯时应该特别注意现做评估。

关键词: 淡色库蚊, 抗药性监测, 容器类型, 杀虫剂

Abstract: Objective　To investigate the influence of containers on the insecticide resistance bioassay among Culex pipiens pallens larvae, and to determine whether disposable cups can replace glass beakers in these tests with the purpose of increasing experimental efficiency. Methods　Using wild Cx. pipiens pallens larvae, the susceptibility of larvae (LC50 with 95%CI) to deltamethrin, temephos, and BMPC was determined using the glass beaker, disposable plastic cup, or disposable paper cup, with impregnation method as recommended by WHO, and the LC50 values measured using different containers were compared. Results　The LC50 (95%CI) values of Cx. pipiens pallens larvae to deltamethrin measured using the glass beaker, disposable plastic cup, and disposable paper cup were 0.002 009 (0.001 654-0.002 449), 0.003 858 (0.003 062-0.005 224), and 0.005 459 (0.004 051-0.008 757) mg/L, respectively; there were significant differences in LC50 value between the glass beaker group and the other two groups. To temephos, the LC50 (95%CI) values were 0.000 955 (0.000 816-0.001 094), 0.003 007 (0.002 661-0.003 407), and 0.003 830 (0.003 450-0.004 256) mg/L, respectively; there were significant differences between the glass beaker group and the other two groups. To BMPC, the LC50 (95%CI) values were 0.3470 (0.3059-0.3821), 0.2575 (0.2118-0.3095), and 0.4844 (0.4460-0.5232) mg/L, respectively; there was a significant difference between the glass beaker group and disposable paper cup group, but no significant difference was found between the glass beaker group and disposable plastic cup group. The susceptibility of Cx. pipiens pallens larvae to deltamethrin was less concentration-dependent as compared with that to temephos; with increasing temephos concentration, susceptibility became less dependent on different types of containers. Conclusion　In the bioassay of Cx. pipiens pallens larvae’s resistance to insecticide, glass breaker is the best choice. Disposable cups may affect the results due to adsorption of pesticides. Special attention should be paid when using disposable cups instead of the glass beaker, and evaluation should be performed in these situations.

Key words: Culex pipiens pallens, Insecticide resistance monitoring, Container type, Insecticide

中图分类号:

林国松, 张晓越, 刘欣, 孟凤霞. 淡色库蚊抗药性测定中不同容器对测定结果的影响研究[J]. 中国媒介生物学及控制杂志, 2014, 25(5): 385-387.

LIN Guo-song, ZHANG Xiao-yue, LIU Xin, MENG Feng-xia. Influence of containers on insecticide resistance bioassayin Culex pipiens pallens larvae[J]. Chines Journal of Vector Biology and Control, 2014, 25(5): 385-387.

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淡色库蚊抗药性测定中不同容器对测定结果的影响研究

Influence of containers on insecticide resistance bioassayin Culex pipiens pallens larvae

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