探讨基于不同基因设计的巴尔通体特异引物

doi:10.11853/j.issn.1003.4692.2014.03.025

摘要/Abstract

摘要：

巴尔通体是一群革兰染色阴性、营养要求苛刻兼性细胞内寄生的需氧多形杆菌, 呈全球性分布的新发现的病原体, 重要性不断增加。巴尔通体培养较难, 表型特征少, 其鉴定主要根据基因扩增。合适的引物尤为重要。在巴尔通体检测、鉴定和种系发生研究中, 设计和应用引物较多, 主要基于16S rDNA、 16S～23S rRNA ITS、gltA、rpoB、ribC、groEL和ftsZ基因等。该文就几种常被选择基因及所设计的引物在巴尔通体研究中的运用做一综述。

关键词: 巴尔通体, 基因, 引物, 序列

Abstract:

Bacteria of the genus Bartonella are Gram?negative, fastidious, aerobic, intracellular parasitic, and pleiomorphic bacillus. It is considered as an emerging pathogen with increasing importance and worldwide distribution. Since it is hard to culture, and the phenotypic characteristics are not clear, identification mainly relies on results of gene amplification. Therefore, suitable primers are particularly important. In detection, identification, and phylogenetic study of Bartonella, many primes are designed and used, mainly based on 16S rDNA, 16S-23S rRNA ITS, gltA, rpoB, ribC, groEL, and ftsZ, etc. In this paper, we review several commonly used genes and primers used in studies of Bartonella.

Key words: Bartonella, Gene, Primer, Sequence

中图分类号:

Q939.93

黄起翡, 李平航, 危玲. 探讨基于不同基因设计的巴尔通体特异引物[J]. 中国媒介生物学及控制杂志, 2014, 25(3): 284-288.

HUANG Qi-fei, LI Ping-hang, WEI Ling. Investigation of Bartonella specific primers designed basedon different genes[J]. Chines Journal of Vector Biology and Control, 2014, 25(3): 284-288.

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