山东省部分地区中华按蚊的抗药性监测

doi:10.11853/j.issn.1003.4692.2014.03.006

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (3): 219-221.DOI: 10.11853/j.issn.1003.4692.2014.03.006

山东省部分地区中华按蚊的抗药性监测

代玉华^{1, 2}, 程鹏¹, 刘丽娟¹, 王海防¹, 公茂庆¹

1 山东省医学科学院, 山东省寄生虫病防治研究所, 山东济宁 272033;
2 河南出入境检验检疫局, 河南郑州 450003

收稿日期:2013-12-23 出版日期:2014-06-20 发布日期:2014-06-20
通讯作者: 公茂庆, Email: maoqingg@yahoo.com
作者简介:代玉华, 女, 博士, 从事媒介生物学研究。Email: dai_yuhua@yahoo.com.cn
基金资助:
国家自然科学基金(81201317, 81201318); 山东省优秀中青年科学家科研奖励基金(bs2011SW032)

Detection investigation of Anopheles sinensis resistance to four commonly used insecticides in selected areas of Shandong province, China

DAI Yu-hua^{1, 2}, CHENG Peng¹, LIU Li-juan¹, WANG Hai-fang¹, GONG Mao-qing¹

1 Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Jining 272033, Shandong Province, China;
2 Henan Entry?Exit Inspection and Quarantine Bureau, Zhengzhou 450003, Henan Province, China

Received:2013-12-23 Online:2014-06-20 Published:2014-06-20
Contact: GONG Mao-qing, Email: maoqingg@yahoo.com

摘要/Abstract

摘要：

目的检测淄博、青岛、济宁3个地区中华按蚊对DDT、溴氰菊酯、氟氯氰菊酯、马拉硫磷的敏感性, 了解山东省部分地区中华按蚊的抗药性。方法采用WHO区分剂量标准和方法, 观察首只蚊虫击倒时间、蚊虫击倒率和死亡数量, 根据校正死亡率判断群体抗性级别。结果淄博、青岛、济宁3个地区的中华按蚊, 接触4%DDT 60 min后击倒率分别为31.78%、22.32%和25.66%, 24 h恢复后的死亡率分别为34.58%、30.40%和24.67%, 均为抗性级别(R);接触0.05%溴氰菊酯60 min后的击倒率分别为40.20%、42.16%和22.73%, 24 h恢复后的死亡率分别为86.58%、61.85%和31.94%, 淄博地区的中华按蚊对溴氰菊酯形成初步抗性(M), 青岛和济宁市的中华按蚊为抗性级别(R);接触0.15%氟氯氰菊酯60 min后的击倒率分别为14.29%、30.10%和13.00%, 24 h恢复后的死亡率分别为66.39%、42.77%和22.05%, 均为抗性级别(R);接触5%马拉硫磷60 min后的击倒率分别为31.19%、49.53%和29.52%, 24 h恢复后的死亡率分别为75.09%、87.18%和81.47%, 淄博地区的中华按蚊对马拉硫磷为抗性级别(R), 青岛和济宁市的中华按蚊形成初步抗性(M)。结论山东省部分地区的中华按蚊对DDT和氟氯氰菊酯仍有较高的抗药性, 对溴氰菊酯和马拉硫磷也有一定的抗药性和抗击倒力。在蚊媒监测与卫生灭蚊工作中, 应根据不同地区蚊虫的抗药性特点采取合理的防治措施, 加强蚊媒与蚊传疾病的监测预警。

关键词: 中华按蚊, 杀虫剂, 抗药性

Abstract:

Objective To determine the sensitivity of Anopheles sinensis to DDT, deltamethrin, cyfluthrin, and malathion in Zibo, Qingdao, and Jining of Shandong province, China. Methods Anopheles sinensis collected were selected and exposed to insecticide?impregnated papers with discriminating concentrations of DDT, deltamethrin, cyfluthrin, and malathion using WHO standard assays. Knock?down rates were recorded at 10, 20, 30, 40, 50, and 60 min of exposure. Mortality resulted from 24 h of exposure was recorded. Resistance was graded according to adjusted mortality rate. Results After exposure to 4% DTT for 60 min, the knock?down rates of An. sinensis in Zibo, Qingdao, and Jining were 31.78%, 22.32%, and 25.66%, respectively. The mortality rates at 24 h after exposure were 34.58%, 30.40%, and 24.67%, respectively. The resistance level of An. sinensis to DDT in the three areas was evaluated as “R”. After exposure to 0.05% deltamethrin for 60 min, the knock?down rates of An. sinensis in Zibo, Qingdao, and Jining were 40.20%, 42.16%, and 22.73%, respectively. The mortality rates at 24 h after exposure were 86.58%, 61.85%, and 31.94%, respectively. The resistance level of An. sinensis to deltamethrin was evaluated as “M” in Zibo and “R” in Qingdao and Jining. After exposure to cyfluthrin for 60 min, the knock?down rates of An. sinensis in Zibo, Qingdao, and Jining were 14.29%, 30.10%, and 13.00%, respectively. The mortality rates at 24 h after exposure were 66.39%, 42.77%, and 22.05%, respectively. The resistance level of An. sinensis to cyfluthrin in the three areas was evaluated as “R”. After exposure to malathion for 60 min, the knock?down rates of An. sinensis in Zibo, Qingdao, and Jining were 31.19%, 49.53%, and 29.52%, respectively. The mortality rates at 24 h after exposure were 75.09%, 87.18%, and 81.47%, respectively. The resistance level of An. sinensis to malathion was evaluated as “R” in Zibo and “M” in Qingdao and Jining. Conclusion The resistance of An. sinensis to DDT and cyfluthrin remains high in some areas of Shandong. The resistance and anti?knockdown potential of An. sinensis to deltamethrin and malathion are also high. The status of insecticide resistance in different areas of Shandong province should be further studied in order to optimize surveillance and management programs.

Key words: Anopheles sinensis, Insecticide, Insecticide resistance

中图分类号:

代玉华, 程鹏, 刘丽娟, 王海防, 公茂庆. 山东省部分地区中华按蚊的抗药性监测[J]. 中国媒介生物学及控制杂志, 2014, 25(3): 219-221.

DAI Yu-hua, CHENG Peng, LIU Li-juan, WANG Hai-fang, GONG Mao-qing. Detection investigation of Anopheles sinensis resistance to four commonly used insecticides in selected areas of Shandong province, China[J]. Chines Journal of Vector Biology and Control, 2014, 25(3): 219-221.

参考文献

[1] Chareonviriyaphap T, Bangs MJ, Ratanatham S. Status of malaria in Thailand[J]. Southeast Asian J Trop Med Public Health, 2000, 31(2):225-237.
[2] Ma Y, Yang M, Fan Y, et al. Population structure of the malaria vector Anopheles sinensis (Diptera: Culicidae) in China: two gene pools inferred by microsatellites[J]. PLoS One, 2011, 6(7):e22219.
[3] Sinka M, Bangs M, Manguin S, et al. The dominant Anopheles vectors of human malaria in the Asia?Pacific region: occurrence data, distribution maps and bionomic precis[J]. Parasit Vectors, 2011, 4:89.
[4] 甄天民, 胡玉祥, 樊天宝. 山东省不同地区的蚊类分布[J]. 中国寄生虫病防治杂志, 1990, 3(4):319-321.
[5] 代玉华, 刘宏美, 刘丽娟, 等. 山东省部分地区蚊虫种类与分布的变化[J]. 寄生虫与医学昆虫学报, 2012, 19(2):84-88.
[6] World Health Organization. Test procedures for insecticide resistance monitoring in malaria vectors bioefficacy and persistence of insecticides on treatedsurfaces[R]. Geneva:WHO, 1998.
[7] Cui F, Raymond M, Qiao CL. Insecticide resistance in vector mosquitoes in China[J]. Pest Manag Sci, 2006, 62:1013-1022.
[8] Kim H, Baek JH, Lee WJ, et al. Frequency detection of pyrethroid resistance allele in Anopheles sinensis populations by real?time PCR amplification of specific allele (rtPASA)[J]. Pestic Biochem Physiol, 2007, 87(1):54-61.
[9] World Health Organization. Malaria control today: current WHO recommendations[R]. Geneva:Working Document March, WHO, 2005.
[10] Sexton JD. Impregnated bed nets for malaria control: biological success and social responsibility[J]. Am J Trop Med Hyg, 1994, 50(6 Suppl):S72-81.
[11] Zuzi L, Mancheng Z, Yuguang W, et al. Trial of deltamethrin impregnated bed nets for the control of malaria transmitted by Anopheles sinensis and Anopheles anthropophagus[J]. Am J Trop Med Hyg, 1989, 40(4):356-359.
[12] Cui F, Tan Y, Qiao CL. Filariasis vector in China: insecticide resistance and population structure of mosquito Culex pipiens complex[J]. Pest Manag Sci, 2007, 63(5):453-458.
[13] World Health Organization. World malaria report 2008 (WHO reference number: WHO/HTM/GMP/2008.1)[R]. Geneva:WHO, 2008. Summary and Key points.
[14] Kawada H, Dida GO, Ohashi K, et al. Multimodal pyrethroid resistance in malaria vectors, Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus s.s. in Western Kenya[J]. PLoS One, 2011, 6(8):e22574.
[15] Ranson H, Abdallah H, Badolo A, et al. Insecticide resistance in Anopheles gambiae: data from the first year of a multi?country study highlight the extent of the problem[J]. Malaria J, 2009, 8:299.
[16] Zhong DB, Chang XL, Zhou GF, et al. Relationship between knockdown resistance, metabolic detoxification and organismal resistance to pyrethroids in Anopheles sinensis[J]. PLoS One, 2013, 8(2):e55475.

山东省部分地区中华按蚊的抗药性监测

Detection investigation of Anopheles sinensis resistance to four commonly used insecticides in selected areas of Shandong province, China

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	赵春春, 周欣欣, 李文玉, 伦辛畅, 刘小波, 吴海霞, 王君, 刘起勇, 孟凤霞. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 30-37.
[2]	郑宇婷, 杨春梅, 杨明东, 姜进勇. 云南边境地区登革热媒介伊蚊生态学及抗药性监测[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 38-43.
[3]	涂涛田, 肖汉森, 孟凤霞, 刘起勇, 刘小波, 何亚明, 季恒青. 重庆市2019年登革热暴发疫情应急控制后媒介伊蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 44-47.
[4]	吴治明, 褚宏亮. 江苏省城市环境中蚊虫越冬状况调查研究[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 83-88.
[5]	吕文祥, 程鹏, 彭荟, 王海洋, 王海防, 郭秀霞, 张崇星, 刘宏美, 公茂庆, 刘丽娟. 山东省东平湖地区2021年淡色库蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 104-107.
[6]	董昊炜, 南春燕, 周秋明, 袁浩, 李翔宇, 彭恒, 马雅军. 基于16S rRNA高通量测序的现场中华按蚊及其孳生地水体菌群结构研究[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 533-540.
[7]	赵琼瑶, 邱星辉, 杨研, 罗兴, 宋晓明, 贾永朝. 四川省广元市中华按蚊杀虫剂靶标基因的多态性及抗性突变频率研究[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 541-545.
[8]	胡远峰, 谭梁飞. 湖北省鄂州市2016－2020年蜚蠊密度及抗药性监测结果分析[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 599-603.
[9]	陈晓敏, 刘芹, 周良才, 吴丽群, 包继永, 吴太平. 武汉市2009－2015年家蝇对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2021, 32(4): 468-471.
[10]	李峰, 崔士磊, 闫静, 丁新阳. 河南省南阳市德国小蠊对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2021, 32(4): 472-474.
[11]	曾旭灿, 许翔, 杨锐, 魏春, 吴林波, 罗春海, 孙晓东. 云南省中华按蚊种群mtDNA-COⅠ基因序列的遗传结构分析[J]. 中国媒介生物学及控制杂志, 2021, 32(3): 265-270.
[12]	王丹, 史鹏, 赵文平, 闫英, 周敬祝, 田珍灶, 梁文琴. 贵州省兴义及赤水市白纹伊蚊抗药性监测分析[J]. 中国媒介生物学及控制杂志, 2021, 32(3): 302-306.
[13]	李春燕, 田丰源, 凌远农, 肖崇堃, 张福艳. 四川省自贡市登革热媒介白纹伊蚊密度监测及其对常用杀虫剂的抗药性调查[J]. 中国媒介生物学及控制杂志, 2021, 32(3): 307-311.
[14]	邹亚明, 兰策介, 朱丁, 游颖琦, 刘蕴华, 陈恩品. 江苏省无锡市2010－2018年德国小蠊对常用杀虫剂的抗药性发展趋势分析[J]. 中国媒介生物学及控制杂志, 2021, 32(2): 193-196.
[15]	章灿明, 李玉伟, 陈斌, 黄婧雯, 黄志余. 福建省2019年国家级监测点白纹伊蚊密度与抗药性监测分析[J]. 中国媒介生物学及控制杂志, 2021, 32(1): 65-69.