Chines Journal of Vector Biology and Control ›› 2013, Vol. 24 ›› Issue (5): 473-476.DOI: 10.11853/j.issn.1003.4692.2013.05.030

• Expert Forum • Previous Articles    

Application of RNA interference in research on functional genes of Blattella germanica

ZHOU Xiao-jie, QIAN Kun, TONG Ying, ZENG Xiao-peng   

  1. Beijing Center for Disease Control and Prevention, Beijing 100013, China
  • Received:2013-04-22 Online:2013-10-20 Published:2013-10-20
  • Supported by:

    Supported by the General Financial Grant from the China Postdoctoral Science Foundation (No. 2013M530727)

RNA干扰技术在德国小蠊功能基因研究中的应用

周小洁, 钱坤, 佟颖, 曾晓芃   

  1. 北京市疾病预防控制中心消毒与有害生物防制所, 北京 100013
  • 通讯作者: 曾晓芃, Email: zengxp@yahoo.cn
  • 作者简介:周小洁(1981- ),男,博士,助理研究员,从事媒介昆虫毒理与分子生物学研究。Email: zhouxj3@163.com
  • 基金资助:

    中国博士后科学基金(2013M530727)

Abstract:

German cockroach (Blattella germanica), the dominant species of cockroaches in China, has an important role in research due to its significance and harmfulness in medicine and economics and being a preponderant research model for many scientific problems. Functional gene research has been the basis for biological research in the post-genome era. RNA interference (RNAi) is a post-transcriptional gene (mRNA) silencing where endogenous or exogenous double-strand RNA specifically and efficiently suppresses the expression of a functional gene at cellular and gross levels. In view of the research value of cockroaches and the technical advantage of RNAi, this article briefly reviews the molecular mechanism of RNAi, with an emphasis on the latest research on the functional genes of B. germanica.

Key words: Cockroach, RNA interference, Double-strand RNA, Functional gene

摘要:

德国小蠊是我国蜚蠊的优势种,在医学与经济方面的重要性和危害性,以及作为多种科学问题的重要研究模型决定了其在生物科学研究领域的重要地位,而在后基因组时代对功能基因的研究成为生物学研究的基础。RNA干扰是外源性或内源性双链RNA诱发的在mRNA水平上进行的基因沉默现象,从而阻止某一基因功能在细胞或整体水平上的表达,具有高效性和特异性等优势。鉴于蜚蠊重要研究价值与RNA干扰表现出的技术优势,该文简要介绍RNA干扰技术分子机制,重点对德国小蠊功能基因的最新研究结果进行综述。

关键词: 蜚蠊, RNA干扰, 双链RNA, 功能基因

CLC Number: