Trends in the development of methods for field detection of mosquito-borne viruses

doi:10.11853/j.issn.1003.8280.2023.03.024

Abstract

Abstract: Mosquito-borne viruses are transmitted mainly by mosquitoes during blood feeding. These viruses include more than 100 species and can cause many infectious diseases and seriously threaten human health. The accurate and rapid detection of mosquito-borne viruses in field is an important part of preventing and controlling mosquito-borne diseases. Field detection allows earlier disease prevention and control and helps control the outbreaks and epidemics of mosquito-borne diseases. However, current detection methods mainly target clinical samples and cannot be used for on-site detection of viruses in mosquito samples. This paper reviews the detection methods for mosquito-borne viruses, including the classical isolation and culture, antigen detection, and nucleic acid detection methods, as well as the new technologies developed rapidly in recent years, such as isothermal amplification and clustered regularly interspaced short palindromic repeats-associatied (CRISPR-Cas). The characteristics of these methods are analyzed, and the directions of developing technologies for monitoring mosquito-borne viruses in field are discussed.

Key words: Mosquito-borne arbovirus, Virus detection, Virus isolation and inoculation, Immunological detection, Molecular detection

摘要： 蚊媒病毒是主要由蚊虫吸血传播的病毒,其种类超过100种,可导致多种传染病,严重威胁人类健康。对现场蚊虫样本中蚊媒病毒的准确、快速检测是蚊媒病风险防控的重要环节,能够将疾病防控关口前移,有助于控制蚊媒病的暴发流行。然而,当前的检测方法主要针对的是临床样品,不能满足现场检测蚊虫样本携带病毒的需求。该文综述了蚊媒病毒检测方法,包括经典的分离培养、抗原检测和核酸检测方法,以及近来发展迅速的等温扩增及其联合规律成簇间隔短回文序列（CRISPR）及相关蛋白系统（CRISPR-Cas）等新技术,分析了它们的特点,并探讨了用于现场监测的蚊媒病毒检测技术的发展方向。

关键词: 蚊媒病毒, 病毒检测, 病毒分离接种, 免疫学检测, 分子生物学检测

CLC Number:

R373.3
R115

JIANG Ning, MA Ya-jun, BAI Jie, PENG Heng. Trends in the development of methods for field detection of mosquito-borne viruses[J]. Chinese Journal of Vector Biology and Control, 2023, 34(3): 422-427.

姜宁, 马雅军, 白洁, 彭恒. 蚊媒携带病毒现场检测方法研发趋势探讨[J]. 中国媒介生物学及控制杂志, 2023, 34(3): 422-427.

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