Chinese Journal of Vector Biology and Control ›› 2022, Vol. 33 ›› Issue (1): 148-152.DOI: 10.11853/j.issn.1003.8280.2022.01.027

• Investigation • Previous Articles     Next Articles

An investigation of ticks and tick-borne bacteria in some areas of Guizhou province, China

XIANG Yu-long1, ZHOU Jing-zhu2, LIU Ying2, LIU Ping-tao3, HU Yong1, LIANG Wen-qin1,2   

  1. 1. School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China;
    2. Experimental Center, Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou 550004, China;
    3. Xiuwen Center for Disease Control and Prevention, Guiyang, Guizhou 550299, China
  • Received:2021-09-18 Online:2022-02-20 Published:2022-02-17
  • Supported by:
    Independent Research Project of the State Key Laboratory of Infectious Disease Prevention and Control (No. 2018SKLID305); Science and Technology Fund Project of Guizhou Provincial Health and Family Planning Commission (No. gzwjkj2018-1-066); National Natural Science Foundation of China (No. 81760605)


向昱龙1, 周敬祝2, 刘英2, 刘平涛3, 胡勇1, 梁文琴1,2   

  1. 1. 贵州医科大学公共卫生与健康学院, 环境污染与疾病监控教育部重点实验室, 贵州贵阳 550025;
    2. 贵州省疾病预防控制中心实验中心, 贵州 贵阳 550004;
    3. 修文县疾病预防控制中心, 贵州 贵阳 550299
  • 通讯作者: 梁文琴,
  • 作者简介:向昱龙,男,在读硕士,主要从事蜱传病原体研究工作,
  • 基金资助:

Abstract: Objective To investigate the situation of ticks and tick-borne bacteria in some areas of Guizhou province, China, and to provide a scientific basis for the prevention and control of tick-borne diseases. Methods Parasitic ticks were collected from the body surface of cattle, sheep, and rodents in Xiuwen county, Qinglong county, Xingyi city, Xingren city, Wangmo county, and Jiangkou county of Guizhou province, in April 2017 and April 2019. Morphological identification was performed on the ticks. Some ticks were selected for bacterial culture using blood agar and brain-heart infusion agar. Colonies with different morphologies were selected for bacterial passage and purification, followed by full 16S rDNA amplification and sequencing to identify the species of bacteria. Results A total of 742 on-host ticks belonging to 6 species of 3 genera were collected. Haemaphysalis longicornis was the dominant species, accounting for 69.81% of the total catches, followed by Rhipicephalus microplus, accounting for 23.05%, and the other tick species only accounted for 7.14%. A total of 188 strains of bacteria were acquired from culture, belonging to 28 genera in 3 phyla. Proteobacteria was the dominant phylum, followed by Firmicutes and Actinobacteria. Conclusion In these areas of Guizhou province, tick species are abundant, and they carry varied species of bacteria, including a variety of zoonotic pathogens. In order to prevent ticks from harming people and livestock, the investigation, monitoring, and control of ticks and tick-borne pathogens should be strengthened.

Key words: Tick, Bacterial culture, Purulent infection, Haemaphysalis longicornis, Proteobacteria

摘要: 目的 对贵州省部分地区开展蜱及其携带细菌调查,为深入了解贵州省蜱及蜱传病原体情况和蜱传疾病的防控提供科学依据。方法 2017年4月和2019年4月在贵州省修文县、晴隆县、兴义市、兴仁市、望谟县、江口县的牛、羊、鼠体表捕捉寄生蜱,进行形态学鉴定;选取部分蜱,采用血平板和脑心浸液培养基(BHI)平板进行细菌培养,挑选不同菌落形态的细菌进行传代纯化,经PCR扩增16S rDNA全长序列,测序鉴定菌种。结果 共采集动物体表蜱3属6种742只,其中长角血蜱为优势蜱种,占捕获总数的69.81%;其次是微小扇头蜱,占比为23.05%;其余蜱种较少,占7.14%。共培养出细菌188株,隶属3门28属,其中变形菌门为优势菌门,其次是厚壁菌门和放线菌门。结论 贵州省调查地区蜱种丰富,蜱体内携带细菌种类繁多,含多种人兽共患病菌。为防止蜱侵害人及家畜,应加强蜱及蜱传病原体的调查、监测与防控。

关键词: 蜱, 细菌培养, 化脓性感染, 长角血蜱, 变形菌门

CLC Number: