Chines Journal of Vector Biology and Control ›› 2021, Vol. 32 ›› Issue (5): 594-598.DOI: 10.11853/j.issn.1003.8280.2021.05.017

• Vector Surveillance • Previous Articles     Next Articles

An investigation of Bartonella infection in rodents in the Changbai Mountains in China and its genotype analysis

WANG Zhuo1, WU Qiong2, JIN Mei-song1, XING Yue-peng3, YANG Yi-jun1, WU Yi-min1   

  1. 1. Biosafety Section of Center for Disease Prevent and Control in Northern Theater Command, Shenyang, Liaoning 110034, China;
    2. Centre of Environmental Detection, Changchun Academy of Plan and Design for Urban and Rural, Changchun, Jilin 130021, China;
    3. Liaoning Institute for Food Control, Shenyang, Liaoning 110016, China
  • Received:2021-03-28 Online:2021-10-20 Published:2021-10-20
  • Supported by:
    Supported by the National Science and Technology Major Project of China (No. 2016ZX10004203-001-009) and the Project of Applied Basic Reseach (No. AWS11L009)


王卓1, 吴琼2, 金美松1, 邢悦鹏3, 杨义军1, 吴益民1   

  1. 1. 北部战区疾病预防控制中心生物安全科, 辽宁沈阳 110034;
    2. 长春市城乡规划研究院环境检测中心, 吉林 长春 130021;
    3. 辽宁省食品检验检测院, 辽宁 沈阳 110016
  • 通讯作者: 杨义军,
  • 作者简介:王卓,女,硕士,副研究员,主要从事病原微生物学检验工作,;吴琼,女,博士,高级工程师,主要从事微生物学研究工作,
  • 基金资助:

Abstract: Objective To investigate the infection and genotype distribution of Bartonella in the rodents in the Changbai Mountains in China. Methods Rodents were captured in Kuandian,forest regions in Ji'an, and arable lands in Mishan in the Changbai Mountains;the rodents were tested for Bartonella rpoB DNA by PCR and the positive amplification products were sequenced and underwent phylogenetic analysis. Results In the 178 rodent spleen specimens tested,77 were positive,with a positive rate of Bartonella of 43.26%. The positive rates in the wild rodents were 66.15%,46.15%,and 8.33%,respectively,in Kuandian,Ji'an,and Mishan,with statistical differences in the infection rate of Bartonella between the three regions (χ2=37.957,P <0.001). Among different rodent species,the positive rates of Bartonella were 16.67%, 33.33%, 50.00%, and 26.67%, respectively, for Rattus norvegicus, Apodemus peninsulae, A. agrarius, and Cricetulus triton,with a statistical difference in the infection rate between the four species (χ2=8.420,P=0.038). Sequencing and phylogenetic analysis showed that four species of Bartonella (B. grahamii, B. taylorii, B. phoceensis, and B. fuyuanensis) were detected from the rodents in the Changbai Mountains. Conclusion Bartonella infection is common in rodents in the Changbai Mountains,which is characterized by genotype diversity and host diversity of Bartonella.

Key words: Bartonella, Rodent, Polymerase chain reaction, Phylogenetic analysis

摘要: 目的 了解我国长白山地区啮齿动物巴尔通体(Bartonella)感染及型别分布情况。方法 对长白山地区宽甸县、集安市林区及密山县耕地捕获的啮齿动物,采用PCR方法检测巴尔通体rpoB (RNA聚合酶亚单位) DNA,阳性扩增产物进行测序和遗传进化分析。结果 PCR检测鼠脾标本共178份,阳性77份,巴尔通体感染率为43.26%。宽甸县、集安市和密山县野鼠感染率分别为66.15%、46.15%和8.33%,各地啮齿动物巴尔通体感染率差异有统计学意义(χ2=37.957,P<0.001)。不同鼠种间,褐家鼠、大林姬鼠、黑线姬鼠和大仓鼠巴尔通体感染率分别为16.67%、33.33%、50.00%和26.67%,感染率差异亦有统计学意义(χ2=8.420,P=0.038)。序列分析与遗传进化分析表明长白山地区啮齿动物中共检出格拉汉姆巴尔通体、泰勒巴尔通体、菲西巴尔通体和抚远巴尔通体4种巴尔通体。结论 长白山地区啮齿动物中巴尔通体感染普遍,存在巴尔通体基因型别的多样性和宿主多样性特征。

关键词: 巴尔通体, 啮齿动物, 聚合酶链式反应, 遗传进化分析

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