Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (4): 354-357.DOI: 10.11853/j.issn.1003.8280.2016.04.010

• Original reports • Previous Articles     Next Articles

Analysis of cytochrome C oxidase subunitⅠgene of unknown and damaged fly species captured at Henan port

ZHANG Qin1, WANG Xiao-meng1, YUE Qiao-yun2, QIU De-yi2, GAO Jie1, ZHANG Fang1, WANG Kai1   

  1. 1 Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, Henan Province, China;
    2 Zhongshan Entry-Exit Inspection and Quarantine Bureau
  • Received:2016-04-11 Online:2016-08-20 Published:2016-08-20

河南口岸截获未知蝇种细胞色素C氧化酶亚基Ⅰ基因分析

张勤1, 王晓梦1, 岳巧云2, 邱德义2, 高洁1, 张芳1, 王凯1   

  1. 1 河南出入境检验检疫局, 郑州 450003;
    2 中山出入境检验检疫局, 广东 中山 528403
  • 作者简介:张勤,女,副主任技师,从事医学媒介生物条形码鉴定及其携带病原体检测工作,Email:qinzhang523@126.com

Abstract:

Objective To identify the unknown fly species with severely damaged morphological characteristics intercepted from Henan port. Methods DNA barcoding based on cytochrome C oxidase subunitⅠ gene was used to amplify and analyze the DNA of the unknown fly species. Results DNA barcoding and sequences analysis showed that the unknown fly species were identified as Chrysomya rufifacies. Conclusion DNA barcoding can partly compensate for the limitations of traditional morphological identification and has broad application prospects in the identification of medical vectors.

Key words: DNA barcoding, Molecular identification, Chrysomya rufifacies

摘要:

目的 应用DNA条形码技术对河南口岸截获的1只形态不完整的未知蝇类进行鉴定。方法 利用以细胞色素C氧化酶亚基Ⅰ(COⅠ)为目的基因的DNA条形码技术,对该未知蝇类基因组DNA进行扩增、序列比对和分析。结果 通过DNA条形码鉴定和序列比对,该未知蝇类的DNA条形码序列与BOLD中绯颜裸金蝇的序列同源性高达100%,确认该蝇种为绯颜裸金蝇。结论 DNA条形码技术弥补了传统形态学鉴定的局限性,在国境口岸医学媒介生物鉴定中有广阔的应用前景。

关键词: DNA条形码, 分子鉴定, 绯颜裸金蝇

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