Chines Journal of Vector Biology and Control ›› 2014, Vol. 25 ›› Issue (5): 464-465.DOI: 10.11853/j.issn.1003.4692.2014.05.022

• Investigation • Previous Articles     Next Articles

Species identification of an imported rodent sample of Bandicota indicaby morphological and genetic analysis

HU Qun, MA Si-jie, MA Yan, MEI Yong   

  1. Daxie Exit-Entry Inspection and Quarantine Bureau, Ningbo 315812, Zhejiang Provinca, China
  • Received:2014-08-12 Online:2014-10-20 Published:2014-10-20

一例输入性板齿鼠的种类鉴定与分析

胡群, 马思杰, 马研, 梅勇   

  1. 大榭出入境检验检疫局,浙江 宁波 315812
  • 作者简介:胡群,男,硕士,高级工程师,主要从事口岸医学媒介生物与病原微生物检测研究,Email: nbhuqun@sina.com

Abstract: Objective To identify the species of a rodent sample captured in an imported container at the port of Zhejiang province, China. Methods Morphological methods and DNA barcoding were applied for rodent identification. Results The rodent had dark-brown fur with an approximate body length of 252 mm, a tail length of 220 mm, and a hind foot length of 40 mm. Genetic analysis of the mitochondrial cytB gene showed that the rodent shared the highest sequence homology with Bandicota indica and matched different individuals at a genetic distance of 0.048 to 0.175. Conclusion Based on morphological characterization and DNA barcode comparison, the imported rodent sample was identified as B. indica. This species was captured for the first time at the port of Zhejiang province, which provided a reference to exclude invasion of exotic disease vectors.

Key words: Bandicota indica, Species identification, Mitochondrial cytochrome B gene, Port

摘要: 目的 对1份从入境集装箱中检获的鼠样品进行种类鉴定。方法 运用形态学方法及DNA条形码技术进行鉴定分析。结果 鼠样品体长约252 mm,尾长220 mm,后足长40 mm,毛色呈黑褐色, cytB基因序列与板齿鼠同源性最为接近,与不同板齿鼠的遗传距离为0.048~0.175。结论 经形态鉴定和DNA条形码比对,确定为板齿鼠,系浙江省口岸首次检获该鼠种,为加强外来医学媒介生物防控提供了基础依据。

关键词: 板齿鼠, 种类鉴定, 线粒体细胞色素B基因, 口岸

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