ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Analysis of the mitochondria gene NADH dehydrogenase 1 in Echinococcus multilocularis at Alataw pass, China-Kazakhstan
Received date: 2016-04-22
Online published: 2016-10-20
Supported by
Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject (No. 2016IK264)
Objective In order to analyze the NADH dehydrogenase subunit 1 (ND1) gene of Echinococcus multilocularis from the wild rodents at Alataw pass, China-Kazakhstan. Methods The ND1 genes of E. multilocularis were amplified by PCR. The sequence was determined by Blast and phylogenetic analysis by Mega 6.0. The transmembrane domain (TM), signal peptide and B cell epitope of the ND1 genes of E. multilocularis were predicted and analyzed by DNAStar. Results The positive rate of E. multilocularis was 1.57%. The results showed that the ND1 genes of E. multilocularis had a length of 894 bp, encoding 297 amino acids. E. multilocularis were 100% closely related to the E. multilocularis AB018440 and AB720065. Conclusion The E. multilocularis can infect wild rodents at Alataw pass. The sequence information of E. multilocularis provides a basis for the scientific monitoring.
YIN Xiao-ping, WANG An-dong, TIAN Yan-he, LIANG Zhen, BA Te, ZHANG Jiang-guo . Analysis of the mitochondria gene NADH dehydrogenase 1 in Echinococcus multilocularis at Alataw pass, China-Kazakhstan[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(5) : 481 -483 . DOI: 10.11853/j.issn.1003.8280.2016.05.015
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