Chines Journal of Vector Biology and Control ›› 2016, Vol. 27 ›› Issue (5): 481-483.DOI: 10.11853/j.issn.1003.8280.2016.05.015

• Original reports • Previous Articles     Next Articles

Analysis of the mitochondria gene NADH dehydrogenase 1 in Echinococcus multilocularis at Alataw pass, China-Kazakhstan

YIN Xiao-ping1, WANG An-dong1,2, TIAN Yan-he1, LIANG Zhen1, BA Te1, ZHANG Jiang-guo1   

  1. 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Bole 833418, Xinjiang Uygur Autonomous Region, China;
    2 College of Animal Science and Technology, Shihezi University
  • Received:2016-04-22 Online:2016-10-20 Published:2016-10-20
  • Supported by:

    Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject (No. 2016IK264)

阿拉山口口岸地区多房棘球蚴线粒体NADH脱氢酶亚基1基因序列分析

尹小平1, 王安东1,2, 田延河1, 梁臻1, 巴特1, 张江国1   

  1. 1 阿拉山口出入境检验检疫局, 新疆 博乐 833418;
    2 新疆石河子大学动物科技学院, 新疆 石河子 832000
  • 作者简介:尹小平,男,副主任医师,从事卫生检疫及鼠防工作,Email:yxpciq@163.com
  • 基金资助:

    国家质检总局科技计划项目(2016IK264)

Abstract:

Objective In order to analyze the NADH dehydrogenase subunit 1 (ND1) gene of Echinococcus multilocularis from the wild rodents at Alataw pass, China-Kazakhstan. Methods The ND1 genes of E. multilocularis were amplified by PCR. The sequence was determined by Blast and phylogenetic analysis by Mega 6.0. The transmembrane domain (TM), signal peptide and B cell epitope of the ND1 genes of E. multilocularis were predicted and analyzed by DNAStar. Results The positive rate of E. multilocularis was 1.57%. The results showed that the ND1 genes of E. multilocularis had a length of 894 bp, encoding 297 amino acids. E. multilocularis were 100% closely related to the E. multilocularis AB018440 and AB720065. Conclusion The E. multilocularis can infect wild rodents at Alataw pass. The sequence information of E. multilocularis provides a basis for the scientific monitoring.

Key words: Echinococcus multilocularis, Mitochondria, NADH dehydrogenase subunit 1, Gene, Alataw pass

摘要:

目的 分析阿拉山口口岸多房棘球蚴线粒体NADH脱氢酶亚基1(ND1)基因序列。方法 使用ND1基因Cest引物对2014年捕获鼠类进行多房棘球蚴初筛,对阳性样品扩增ND1基因全长序列,利用Blast分析序列同源性、Mega 6.0软件构建分子遗传进化树,使用DNAStar软件分析其跨膜结构、信号肽、B细胞抗原表位等。结果 阿拉山口口岸1.57%的野鼠感染多房棘球蚴,其线粒体 ND1基因全长894 bp,编码297个氨基酸,与日本的多房棘球蚴AB018440和AB720065同源性最高,为100%。结论 阿拉山口口岸地区野鼠存在多房棘球蚴感染,其序列信息为该地区多房棘球蚴的科学监控提供依据。

关键词: 多房棘球蚴, 线粒体, NADH脱氢酶亚基1, 基因, 阿拉山口口岸

CLC Number: