Abstract: Objective To investigate the resistance level of Aedes albopictus to commonly used insecticides in Weifang,Shandong, China, so as to provide a reference for the scientific use of insecticides.Methods The larval dipping method was used to determine larval deaths, the SPSS 19 software was used to caculate the toxic regression equation and the median lethal concentration (LC₅₀) of insecticides on the larvae at the late 3rd instar and the early 4th instar, and WHO tube method was used to measure the 24 h mortality rate of healthy female mosquitoes without blood-sucking.Results The larvae of Ae. albopictus showed a resistance ratio of 3.07-6.52 fold to permethrin,propoxur,and temephos and a resistance ratio of 12.40-18.40 fold to Bacillus thuringensis israelensis (Bti, 7 000 ITU/mg). Adult mosquitoes developed resistance to 0.08% beta-cypermethrin, 0.4% permethrin,0.07% lambda-cyhalothrin,and 0.03% deltamethrin, with a 24 h mortality rate of 15.94%-62.32%. The mosquitoes were determined as possibly resistant to 0.2% fenitrothion in 2022 (with a mortality rate of 92.65%) and were determined as sensitive to 0.5% malathion, 2% chlorpyrifos, 0.2% fenitrothion, 0.2% bendiocarb, and 0.05% propoxur, with a mortality rate of 98.63%-100%.Conclusions Ae. albopictus mosquitoes in Weifang have developed serious resistance to most pyrethroid insecticides. Therefore,local use of insecticides for the prevention and control of mosquitoes should be adjusted according to surveillance results of insecticide resistance, and integrated measures, mainly environmental management, should be implemented. In addition, different insecticides should be used alternatively or in combination, so as to prevent and delay the development of insecticide resistance.

Key words: Aedes albopictus, Larvae dipping method, WHO tube method, Insecticide resistance

摘要： 目的了解潍坊市白纹伊蚊对常用卫生杀虫剂的抗药性,为科学使用杀虫剂提供依据。方法采用幼虫浸渍法,测定白纹伊蚊Ⅲ龄末Ⅳ龄初幼虫死亡数,SPSS 19软件计算毒力回归方程和幼蚊对杀虫剂的半数致死浓度（LC₅₀）；采用成蚊接触筒法,测定羽化后3～5 d未吸血健康雌蚊24 h死亡率。结果白纹伊蚊幼虫对氯菊酯、残杀威和双硫磷抗性倍数为3.07～6.52倍。对苏云金芽孢杆菌以色列亚种（Bti,7 000 ITU/mg）抗性倍数为12.40～18.40倍。成蚊对0.08%高效氯氰菊酯、0.4%氯菊酯、0.07%高效氯氟氰菊酯和0.03%溴氰菊酯均产生抗性,24 h死亡率在15.94%～62.32%；除对0.2%杀螟硫磷2022年测定结果为可能抗性（死亡率为92.65%）外,对0.5%马拉硫磷、2%毒死蜱、0.2%杀螟硫磷、0.2%虫威和0.05%残杀威均表现为敏感,死亡率为98.63%～100%。结论潍坊市白纹伊蚊已对大部分拟除虫菊酯类杀虫剂产生了较为严重的抗药性,需根据监测结果调整当地蚊虫防治药剂使用,且应采取环境治理为主的综合性防治措施,不同作用机制杀虫剂交替轮用或复配使用,预防和减缓抗药性的产生。

关键词: 白纹伊蚊, 幼虫浸渍法, 成蚊接触筒法, 抗药性