Abstract: Objective To investigate the pathogenic bacteria carried by Blattella germanica in the urban communities of Henan province, China by 16S rRNA sequence analysis. Methods A universal primer for 16S rRNA was designed, and the single colonies isolated from the body surfaces or inside the bodies of B. germanica samples were treated by PCR amplification with the primer. The amplification products were sequenced and then identified using Basic Local Alignment Search Tool (BLAST), and the sequences that shared the highest similarity with the amplification products were selected. Results The identified pathogens included 2 pathogenic bacteria, Shigella flexneri and Salmonella enteria subsp. enterica, 5 conditionally pathogenic bacteria, Enterobacter cloacae, Citrobacter koseri, Klebsiella pneumoniae, Escherichia coli and Serratia sp., and 2 engineering bacteria, Bacillus halodurans and B. amyloliquefaciens. Conclusion In the urban communities of Henan province, B. germanica is a pathogen vector, which cannot be neglected; 16S rRNA sequence analysis can be used as a method for rapid identification of pathogens, which is helpful to vector risk assessment.

Key words: Blattella germanica, Pathogen, 16S rRNA

摘要： 目的 基于16S rRNA序列分析法检测河南省城市居民区中德国小蠊携带致病菌情况。方法 设计通用的16S rRNA基因引物,对从试虫体表或体内分离出的单菌落进行PCR扩增并测序,再利用BLAST序列比对检索系统寻找同源性最高的序列作为鉴定结果。结果 分离出福氏志贺菌和伤寒沙门菌2种病原菌,阴沟肠杆菌、柯氏柠檬酸杆菌、大肠埃希菌、沙雷菌和肺炎克雷伯菌5种条件致病菌,以及嗜碱芽孢杆菌和解淀粉芽孢杆菌2种工程菌。结论 河南省城市居民区中德国小蠊携带致病菌的情况不容忽视;16S rRNA序列分析法可以作为微生物快速鉴定方法为今后的病媒生物防制工作提供帮助。

关键词: 德国小蠊, 致病菌, 16S rRNA