Phylogenetic analysis of rpoB gene of Bartonella in rodents in Zhejiang province

Abstract

Abstract:

Objective To get an insight into the carriage status and epidemic characteristics of Bartonella in rodents in Zhejiang province for providing baseline data for the development of measures for the control and prevention of Bartonella infection. Methods DNA was extracted from the livers and spleens of mice and the beta-subunit-encoding gene (rpoB) of Bartonella was selected for PCR amplification, the positive products of which were sequenced and phylogeneticaly analyzed. Results One of 101 rodents captured in Panan county of Jinhua city in Zhejiang was positive for the rpoB gene of Bartonella with the isolation rate of Bartonella in Rattus tanezumi being 4.3％. Phylogenetic analysis showed that the isolated strain was of the same evolutionary branch as B. grahamii (GenBank accession No. AB426694.1) with a bootstrap value of 96%. Conclusion There exists Bartonella infection in rodents in Zhejiang province with R. tanezumi as its possible host. The isolated strain belongs to B. grahamii.

Key words: Bartonella, rpoB gene, Phylogenetic analysis

摘要：

目的初步了解巴尔通体在浙江省鼠群中携带状况及流行特征，为制定预防措施提供依据。方法 2009年8月于浙江省磐安县捕鼠101只，提取其肝、脾DNA后，选择巴尔通体属rpoB基因进行PCR扩增，并对所得产物进行测序及进一步的系统进化分析。结果 2009年在磐安县捕获的101只鼠中肝脾标本检出巴尔通体rpoB基因阳性1例，其中黄胸鼠中巴尔通体分离率为4.3%; 此分离株与格雷汉巴尔通体(GenBank序列号：AB426694.1)处于同一进化分支上(自展值为96%)。结论在浙江省鼠群中存在巴尔通体感染，分离株种属为格雷汉巴尔通体。

关键词: 巴尔通体, rpoB基因, 进化分析

CLC Number:

Q939.93
S443

ZHAN Li, JIANG Li-ping, LI Zhong-liang, YING Kai-man, MENG Zhen, MEI Ling-ling. Phylogenetic analysis of rpoB gene of Bartonella in rodents in Zhejiang province[J]. Chines Journal of Vector Biology and Control, 2011, 22(2): 151-153.

占利, 姜理平, 李钟梁, 应凯满, 孟真, 梅玲玲. 浙江省鼠中巴尔通体rpoB基因系统进化分析[J]. 中国媒介生物学及控制杂志, 2011, 22(2): 151-153.

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