Abstract:

Objective To determine the status quo of West Nile virus (WNV) infections in animals in Ili, Sinkiang, providing the relevant data for WNV prevention and control in China. Methods One-step real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the WNV envelope protein gene from the brain tissues of 70 donkeys and 100 shepherd dogs collected in Ili, Sinkiang. Results Of the brain samples of 70 donkeys and 100 shepherd dogs, 2 were suspected positive for WNV envelope protein gene fragments. However, they were excluded through 3% gel electrophoresis revalidation. Therefore, all tested samples were negative for the WNV envelope protein gene. Conclusion WNV infection is not found in brain tissues of donkeys and shepherd dogs in Ili, Sinkiang.

Key words: West Nile virus, Fluorescent quantitative RT-PCR

摘要：

目的 了解新疆伊犁地区动物中西尼罗病毒(West Nile virus，WNV)感染现状，为我国WNV的防治提供资料。方法 采用一步法实时荧光定量反转录-聚合酶链反应(RT-PCR)，对采自新疆伊犁地区的70头驴和100只牧羊犬的脑组织进行WNV包膜蛋白(E)基因片段检测。结果 70头驴和100只牧羊犬脑组织标本的西尼罗病毒包膜蛋白基因片段检测有2例可疑阳性，但经3％凝胶电泳法再次验证后排除。故所有待检样本WNV包膜蛋白基因检测均为阴性。结论 我国新疆伊犁地区的驴和牧羊犬脑组织中未检测到WNV的感染。

关键词: 西尼罗病毒, 实时荧光定量RT-PCR