Abstract: In order to study the immune effect and enhancement of inactivated vaccine prepared with golden hamster cells, we examined the serological and epidemical effect of one year after strengthened immunization be fore strengthened immunization and on 14 days and 12 monthes after strengthened irnmunization, the seroconversion rates of neutralizing antithey were 18.5%、 l00%、60%, GMTS were 3.2.17.05.5.75, respectively.There were no patients in the immunized group and 12 patients in the contro group after strengthened immunization, the incidence rate was 101.2/100 000, the protection rate was 100%.Before and after the epidemic peak (September in 1996 and January in 1997), we collected double serum and did not find the natural infection by HV.

摘要： 观察肾综合征出血热（HFRS）地鼠肾细胞Ⅱ型灭活疫苗在以家鼠型为主疫区接种后一年的血清学和防病效果。方法：采用IFAT和MCPENT法检测疫苗接种者静脉血的荧光抗体和中和抗体阳转率与GMT滴度，追踪观察防病效果。结果：（1）加强免疫前、加强免疫后14天及12个月，IFAT阳性率分别为62.06％、100％和56．1％，GMT分别为18.77、98．83、29.22；中和抗体阳转率分别为18．5％、100％和60％，GMT分别为3．2、17．05和5．75。（2）加强免疫后接种组无一病例；对照组发病12例，发病率为101．2／10万，保护率100％。（3）采集接种人群流行高峰期前（9月）、后（翌年1月）双份血，IFAT未检测出汉坦病毒（HV）自然感染者。结论：加强注射后抗体滴度迅速升高，中和抗体和荧光抗体阳转率均达100％。加强免疫后1年，抗体滴度和阳转率均明显下降，但仍有好的防病效果。

关键词: 肾综合征出血热灭活疫苗, 免疫效果, 免疫增强