Abstract: Objective To analyze the expression level of transferrin gene in the susceptible population and resistant population of Culex pipiens quinquefasciatus. Methods RNA was extracted and was amplified by quantitative real-time PCR (qRT-PCR) to produce partial gene fragments of the target gene using the designed primers. After purification and sequencing, the sequence alignment was performed. The t test was used to statistically analyze the expression levels of transferrin gene in the susceptible population and resistant population of Cx. pipiens quinquefasciatus. Results The qRT-PCR analysis showed that the mean fluorescence quantification values of transferring gene in susceptible population and resistant population of Cx. pipiens quinquefasciatus were 15.21 and 18.41, respectively; the mean fluorescence quantification values of the internal reference gene in the two populations were 20.53 and 19.10, respectively. The expression level of transferrin gene in the resistant population of Cx. pipiens quinquefasciatus was reduced, and there was a significant difference in the expression level of transferrin gene between the two populations (t=10.390, P<0.001). Conclusion The transferrin gene of Cx. pipiens quinquefasciatus can be used as a target gene for the detection and treatment of mosquito resistance.

Key words: Culex pipiens quinquefasciatus, Transferrin, Quantitative real-time polymerase chain reaction

摘要： 目的 分析转铁蛋白基因在致倦库蚊敏感品系、抗性种群体内的表达量。方法 设计引物、提取RNA、扩增目的基因部分基因片段、纯化测序、序列比对、荧光定量PCR。采用t检验对敏感品系和抗性种群致倦库蚊体内转铁蛋白的基因表达量进行统计分析。结果 扩增致倦库蚊转铁蛋白部分基因片段，测序后序列比对，荧光定量PCR分析显示，致倦库蚊敏感品系转铁蛋白荧光定量（T_f敏）和抗性种群转铁蛋白荧光定量（T_f抗）平均值分别为15.21和18.41，内参基因荧光定量（β_敏和β_抗）平均值分别为20.53和19.10，转铁蛋白基因在致倦库蚊抗性种群中的表达量降低，2个样本体内转铁蛋白基因表达差异有统计学意义（t=10.390，P<0.001）。结论 致倦库蚊转铁蛋白基因可作为蚊虫抗性检测及治理的靶标基因。

关键词: 致倦库蚊, 转铁蛋白, 荧光定量聚合酶链式反应