Chines Journal of Vector Biology and Control ›› 2014, Vol. 25 ›› Issue (2): 127-130.DOI: 10.11853/j.issn.1003.4692.2014.02.010

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Detection of pathogen infections in parasitic ticks of wild rodentsat Erenhot port

CAO Xiaomei1, ZHANG Xiaolong1, FANG Wei1, QIAO Shun2, LIU Wenjin1, YANG Pengfei1, WEI Huaibo2, TIAN Li2, YAO Lisi1, GUO Tianyu1, YANG Yu1   

  1. 1 Chinese Academy of Inspection and Quarantine Science Research, Beijing 100123, China;
    2 Erenhot Entry?Exit Inspection and Quarantine Bureau
  • Received:2013-12-09 Online:2014-04-20 Published:2014-04-20
  • Contact: YANG Yu, Email: redyy99@gmail.comSupported by the International
  • Supported by:
    Science and Technology Cooperation Program of China (No. 2012DFA30540), National Science and Technology Support Project of China (No. 2012BAK11B05-02) and Major National Science and Technology Projects of China (No. 2012ZX10004219-005)

二连浩特口岸鼠体寄生蜱感染病原体调查

曹晓梅1, 张晓龙1, 房魏1, 乔舜2, 柳文进1, 杨鹏飞1, 魏怀波2, 田丽2, 姚李四1, 郭天宇1, 杨宇1   

  1. 1 中国检验检疫科学研究院, 北京100123;
    2 二连浩特出入境检验检疫局
  • 通讯作者: 杨宇, Email: redyy99@gmail.com
  • 作者简介:曹晓梅, 女, 博士, 副研究员, 从事医学媒介生物学研究。Email: cxm910@126.com
  • 基金资助:
    国家国际科技合作专项(2012DFA30540); 国家科技支撑计划 (2012BAK11B05-02); 国家科技重大专项课题 (2012ZX10004219-005)

Abstract: Objective To investigate the species of ticks parasitizing wild rodents and tick?borne pathogens at Erenhot port. Methods Ticks were collected from captured field rats, and genomic DNA and total RNA were extracted from each tick. PCR and RT?PCR were used to detect Borrelia burgdorferi, Q fever rickettsia, Babesia microti, spotted fever group rickettsia (SFGR), Anaplasma, Russian spring?summer Encephalitis virus, Bunyavirus, and Crimean?Congo hemorrhagic fever virus. Results A total of 152 parasitic ticks were collected, including Haemaphysalis verticalis, Dermacentor niveus, and Hyalomma asiaticum, and H. verticalis was the dominant species, accounting for 98.03% of all captured ticks. Nineteen cases of SFGR were detected among H. verticalis, D. niveus, and H. asiaticum, with a detection rate of 12.50%, but the other seven species of pathogens were not detected. Conclusion There are natural foci of SFGR in the area of Erenhot port.

Key words: Parasitic tick, Tick?borne pathogen, Spotted fever group rickettsia, Erenhot

摘要: 目的 调查二连浩特口岸鼠体寄生蜱种群组成及自然感染病原体情况。方法 从捕获的鼠体上收集寄生蜱, 提取单只蜱DNA基因组及总RNA, 采用PCR或RT?PCR方法检测莱姆病螺旋体、Q热立克次体、巴贝西原虫、斑点热立克次体、无形体、森林脑炎病毒、布尼亚病毒、克里米亚-刚果出血热病毒。结果 共采集鼠体寄生蜱152只, 分别为草原血蜱、银盾革蜱和亚东璃眼蜱, 其中草原血蜱为优势种, 占捕获总数的98.03%。从草原血蜱、银盾革蜱和亚东璃眼蜱中检测到斑点热立克次体19份, 检出率为12.50%, 其他病原体检测均为阴性。结论 二连浩特口岸地区存在斑点热立克次体自然疫源地。

关键词: 蜱类, 蜱媒病原体, 斑点热群立克次体, 二连浩特

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