ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective: To select chemical substances with attractive effects on Aedes albopictus for on-field assessment, and to explore the preference of Ae. albopictus to six human odors. Methods: Under laboratory conditions, six chemical substances (1-octen-3-ol, lactic acid, myristic acid, ammonia, acetone, and ethyl acetate) were separately tested for their individual attracting effects on female Ae. albopictus at concentrations of 0.1, 1.0, and 10.0 mg/ml. The six substances were mixed in pairs (1∶1) at certain concentrations, and then the pairs were tested for attracting effects on female Ae. albopictus. The pair with good attractive effects was combined with lactic acid for testing. The attractant combination selected by laboratory experiments was tested for attractive action for Ae. albopictus in the field. The attracting effects of the substances or combinations on Ae. albopictus were compared using the t test and one-way analysis of variance. Results: Ae. albopictus mosquitoes were attracted to 1-octen-3-ol at 0.1, 1.0, and 10.0 mg/ml, myristic acid at 1.0 and 10.0 mg/ml, and ethyl acetate at 0.1 mg/ml, but not to the other three substances at any concentration. The pairs of 1-octen-3-ol+myristic acid, lactic acid+myristic acid, and lactic acid+1-octen-3-ol statistically attracted Ae. albopictus compared with the control group (t=8.102, P=0.001; t=4.696, P=0.009; t=5.127, P=0.007). The combination of lactic acid+1-octen-3-ol+myristic acid statistically attracted Ae. albopictus compared with the control group (F=86.841, P < 0.001). Field testing showed significant differences in the attracting effect between lactic acid+1-octen-3-ol+myristic acid and the control (day 1: t=7.462, P=0.014; day 2: t=20.500, P < 0.001; day3: t=9.383, P=0.001). Conclusion: The combination of lactic acid, 1-octen-3-ol, and myristic acid has attractive action for Ae. albopictus, which should be further researched in the field.