Objective This paper was intended to analyze the spatiotemporal patterns of the Japanese encephalitis (JE) in Henan province from 2006 to 2010, and explore the connection between JE incidence and the geographical environmental driving factors. Methods The JE incidence from 2006 to 2010 in Henan was analyzed with epidemiological research, spatial autocorrelation, and correlation method. The multiple linear regression model was also built to identify the impact of meteorological, surficial and socio-economic factors on JE. Results The total number of JE case is 2 729 with yearly incidence of 0.547 4 per 100 thousand people and the overall death case was 119 with mortality rate of 4.36%. The incidence outbreak from July to September, accounting for 97.08% of the whole year cases and the annual incidence declined in 2008 and then fluctuated until 2010. JE cases were mainly concentrated in the southwest area, and "hot spots" agglomeration was significant in Luoyang, Nanyang, and northern Xinyang city. In terms of the meteorological driving factors, rainfall, the number of rainy days, >80% humidity days and >25℃ cumulated temperature promoted the outbreak of JE, while >30℃ cumulated days and the maximum above 30℃ cumulated temperature would reduce the incidence. For the surficial factors, watershed density and cultivated area illustrated the significant correlation with incidence of 99% confidence. With the increase of income per capita in farmers and total number of health technical staff, the possibility of an outbreak of JE also decreased. The overall predicted R² of regression model was 0.643 with the adjusted R² of 0.414. Conclusion The southwest area in Henan province is the key area for JE management. Research on the geographical driving factors of JE disease, will have a significant effect on the prevention and control of JE incidence.

Objective To develop a mouse model for exploration of the pathogenesis of Dengue virus (DENV) infection. Methods Human normal hepatic cell line LO2 was transplanted to severe combined immunodeficiency (SCID) mice intraperitoneally to develop a SCID-LO2 chimeric mouse model. Following challenge with intraperitoneally injected DENV, the model was evaluated based on virus distribution and the histological changes of main organs. Results The LO2 cells were successfully engrafted into SCID mice. SCID-LO2 chimeric mice showed viremia and severe organ injury after the infection of DENV, but without hind - leg paralysis. Conclusion SCID-LO2 chimeric mice can maintain the replication of DENV and manifest some clinical symptoms of DENV infection in humans. Therefore, the SCID-LO2 chimeric mouse model is valuable for studying the pathogenic mechanism of DENV infection.

　　【Abstract】　Objective　To grasp the community structure and quantity fluctuation of rat?shape mammals and provide the scientific basis for the evaluation and control of the mammals. Methods　Rat?shape mammals were captured by night trapping method along the washland of the “Three?rivers” in Leshan city from September 2007 to August 2008. Results　There were four species of rate?shape mammals. Among them, Anourosorex squamipes(49.55%) and Apodernus agrarius(45.34%) were the dominant species, Crocidura shantungensis(3.60%) was the common species, and Rattus norvegicus(0.90%) was the rare species. Seen from the quantity fluctuation of rat?shape mammals, there were two peaks in the whole year. One was in July and the other was October, but the peak value of the former was prominently higher than the latter. In winter and spring, the quantity basically maintained a lower level. And then, it began to go up quickly in June. By and large, the quantity fluctuation of the two dominant species also accorded with the rule.  Conclusion　 An.squamipes and Ap.agrarius were the main targets to prevent and control.