Objective To investigate the resistance of Musca domestica to five common insecticides in Wenzhou, China, and to provide a basis for scientific and rational use of insecticides and development of targeted prevention and control measures. Methods From May to October, 2017, M. domestica adults were collected from each monitoring site in Wenzhou and were then brought to the insectary for breeding. The micro-drip method was used to determine the median lethal dose (LD₅₀) of permethrin, deltamethrin, beta-cypermethrin, dichlorvos, and propoxur for M. domestica. Results The LD₅₀ of permethrin, deltamethrin, beta-cypermethrin, dichlorvos, and propoxur was 0.425 3, 0.262 6, 3.281 8, 9.667 9, and >48 μg/♀, respectively. Compared with the LD₅₀ of sensitive lines, the resistance ratios were 39.38, 291.78, 911.61, 213.42, and >161.45 times, respectively. Conclusion Musca domestica in the monitoring sites has developed a high level of resistance to common insecticides, and there has been a significant increase in resistance in recent years. Therefore, scientific management and technical guidance for the use of insecticides should be strengthened.

　　【Abstract】　Objective　To detect the difference of the salivary protein spectrum from Aedes albopictus aged 3 days with blood-feed and sugar-feed, respectively.  Methods　The  salivary  protein was detected with Pierce BCA Protein Assay Kit and SDS-PAGE. Results　The amount of salivary gland proteins in sugar-feed and blood-feed mosquitoes aged 3 days was approximately （1.243±0.460）μg/female and （0.945±0.460）μg/female, respectively.  There were at least twelve major proteins in sugar-feed mosquito salivary glands analyzed by SDS-PAGE, and no difference was detected in the electrophoretic bands between sugar-feed and blood-feed mosquitoes salivary gland. Conclusion　No specific protein was induced after Ae.albopictus feeding blood, but the total protein of salivary gland per female decreased after a blood meal.

Objective To understand the epidemiological characteristic of hemorrhagic fever with renal syndrome(HFRS) in human and animals in Heilongjiang province,and to guide the disease prevention and control.Methods The surveillance data of HFRS in Heilongjiang province in 2006 was collected and analyzed.At the same time the human sera,the rodent lung tissues and sera in the surveillance areas were sampled to detect the antigen and antibody of hantavirus(HV).Results 3870 cases had been reported in Heilongjiang province in 2006,of which,33 cases died.The annual mean morbidity,death rate and case fatality was 10.13/100 000,0.09/100 000 and 0.85% respectively.The case number declined 7.68% in 2006 in comparison with that in 2005.The main attacked areas of HFRS were Jiamusi city,Shuangyashan city,Heihe city and Jixi city.The 10 species of 2554 rodents were caught,and the 2461 rodent lung tissues,2461 rodent sera,598 patient sera and 527 healthy people sera were collected.The further study indicated that the density of rodents was 11.19%,Rattus norvegicus and Apodemus agrarius was the dominant species in human living areas and in fields respectively.The serum positive rate of patients was 63.88%,and the HV recessive infection rate of healthy people was low.The HV carrying rate of rodents was 7.68%,and the antibody positive rate of rodent blood was 10.73%.Conclusion Heilongjiang province belonged to type-mixed HFRS epidemic area where Apodemus species was dominant.Although the epidemic situation of HFRS declined,the density and the HV carrying rate of rodents were still severe,so the surveillance of the epidemic should be reinforced,especially preventive inoculation should be strengthened.