Total protein spectrum of salivary gland from Aedes albopictus aged 3 days after a blood meal

Abstract

Abstract:

　　【Abstract】　Objective　To detect the difference of the salivary protein spectrum from Aedes albopictus aged 3 days with blood-feed and sugar-feed, respectively. Methods　The salivary protein was detected with Pierce BCA Protein Assay Kit and SDS-PAGE. Results　The amount of salivary gland proteins in sugar-feed and blood-feed mosquitoes aged 3 days was approximately （1.243±0.460）μg/female and （0.945±0.460）μg/female, respectively. There were at least twelve major proteins in sugar-feed mosquito salivary glands analyzed by SDS-PAGE, and no difference was detected in the electrophoretic bands between sugar-feed and blood-feed mosquitoes salivary gland. Conclusion　No specific protein was induced after Ae.albopictus feeding blood, but the total protein of salivary gland per female decreased after a blood meal.

Key words: Aedes albopictus, Salivary gland, SDS-PAGE

摘要：

　　【摘要】　目的　探讨3日龄白纹伊蚊雌蚊吸食糖水组和饱血组唾液腺总蛋白谱的差异。方法　分别采用Pierce蛋白定量试剂盒和SDS?PAGE电泳分析白纹伊蚊3日龄吸食糖水组和饱血组成蚊唾液腺蛋白质浓度和蛋白谱。结果　糖水组蚊虫单对唾液腺蛋白质质量约为（1.243±0.460）μg，饱血组约为（0.945±0.460）μg；SDS?PAGE检测到糖水组唾液腺总蛋白有12条主带，糖水组与饱血组唾液腺总蛋白条带没有变化。结论　白纹伊蚊雌蚊饱血后唾液腺总蛋白无特异条带诱导，但饱血后单对唾液腺总蛋白下降。

关键词: 白纹伊蚊, 唾液腺, SDS?PAGE

CLC Number:

R384.1

WU Jia-Hong, SUN Yu, CHENG Jin-Zhi, CHEN Lu. Total protein spectrum of salivary gland from Aedes albopictus aged 3 days after a blood meal[J]. Chines Journal of Vector Biology and Control, 2009, 20(2): 102-104.

吴家红，孙宇，程金芝，陈璐. 白纹伊蚊3日龄成蚊饱血前后唾液腺总蛋白谱[J]. 中国媒介生物学及控制杂志, 2009, 20(2): 102-104.

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