Objective To investigate the influencing factors of dengue fever and the existing problems in prevention and control efforts against dengue fever in the representative areas of Yunnan and Guangdong provinces, China through in-depth interviews, and to put forward targeted recommendations for local prevention and control strategies against dengue fever.Methods Using a self-designed interview outline, one-to-one interviews with 25 professionals engaged in dengue fever prevention and control research, epidemiological investigations, and treatment from the cities and prefectures with severe dengue fever outbreaks in Yunnan and Guangdong provinces were conducted. Electronic interview documents were obtained and imported into MAXQDA 2020 software for thematic framework analysis, including categorization, coding, analysis, and charting.Results Through the analysis of the interview data, a total of four first-level themes were obtained: (1) the current situation of dengue fever prevention and control, i.e., the current prevention and control strategies and measures, which were summarized in four measures attributed to routine response and seven to response strategies and measures against outbreaks; (2) factors affecting dengue fever prevention and control, including two climatic factors and six social factors, among which social factors could be controlled through human intervention to control the spread of dengue fever; (3) problems in dengue fever prevention and control, including inadequate government’s attention, unbalanced and insufficient financial investment, low public participation, and a lack of assessment and supervision mechanisms, which were most frequently mentioned by experts, along with six other problems, according to the interview data; (4) key points of response to dengue fever, which were summarized in ten key points of response.Conclusions The representative areas of dengue fever in Yunnan and Guangdong provinces should establish a long-term response mechanism led by the government with multi-sectoral cooperation to fully mobilize residents to participate in prevention and control, reasonably allocate funds for prevention and control, strengthen the control of Aedes vectors, and provide a scientific basis for the sustainable and precise control of dengue fever and other Aedes vector-borne diseases in China.
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