Objective To establish the sensitive baseline of houseflies to six commonly used insecticides, so as to provide a basis for the monitoring and control of the insecticide resistance of houseflies.Methods The topical application method was used to test the sensitivity of laboratory susceptible housefly strains to six insecticides (deltamethrin, beta-cypermethrin, permethrin, dichlorvos, propoxur, and dinotefuran) of four categories. Data from previous literature were collected for comparison. The statistical significance of sensitivity differences was determined according to whether the 95% confidence interval of the median lethal dose (LD50) overlapped. Based on the above steps, the baseline sensitivity of houseflies to the six insecticides was established.Results The baseline sensitivity of the six test insecticides against houseflies were as follows: deltamethrin (LD50=0.002 236 μg/♀, y=13.76+5.19x), beta-cypermethrin (LD50=0.003 692 μg/♀, y=9.34+3.84x), permethrin (LD50=0.007 152 μg/♀, y=10.03+4.67x), dichlorvos (LD50=0.074 20 μg/♀, y=5.02+4.44x), propoxur (LD50=0.415 9 μg/♀, y=0.93+2.43x), and dinotefuran (LD50=0.103 6 μg/♀, y=6.31+6.41x).Conclusion This study established the baseline sensitivity of houseflies through testing the sensitivity of laboratory susceptible strains to six commonly used insecticides, providing a reference for monitoring and assessing the resistance level of houseflies in China.
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