Objective To monitor five common pathogens carried by small mammals in key areas of Chongqing, China, so as to provide a scientific basis for the prevention and control of local rodent-borne diseases.Methods From 2021 to 2022, small mammals were collected using cage traps at night at three surveillance sites in the central urban area, Wanzhou District, and Fuling District of Chongqing. The liver, spleen, lung, and kidney tissues were collected to extract nucleic acids for detection of Leptospira interrogans, Rickettsia mooseri, and Bartonella by quantitative real-time PCR (qPCR) as well as Hantavirus and Dabie bandavirus by reverse transcription qPCR. Excel 2010 was used to sort and arrange the data. The SPSS 22.0 software performed statistical analysis on the experimental data. The species composition ratio and pathogen infection rate of rodents were compared by the Chi-squared test.Results A total of 1 188 small mammals were collected, among which Rattus norvegicus accounted for 33.50%, R. tanezumi accounted for 26.77%, Anourosorex squamipes accounted for 26.01%, and Mus musculus accounted for 9.85%, in addition to Leopoldamys edwardsi, R. nitidus, N. confucianus, Apodemus agrarius, and Crocidura attenuata, with a total of nine species. There was a significant difference in the species composition of small mammals between different surveillance sites (χ2=714.786, P<0.001). Four samples were positive for Seoul Hantavirus, 24 for L. interrogans, and 8 for Bartonella; the remaining samples were negative. A total of 33 small mammals were found positive, including 2 R. tanezumi and 1 R. norvegicus carrying both L. interrogns and Seoul Hantavirus. The overall infection rate was 2.78% for all pathogens, 0.34% for Hantavirus, 2.02% for L. interrogans, and 0.67% for Bartonella. There was a significant difference in the infection rates of the three pathogens (χ2=18.857, P<0.001).Conclusions L. interrogans, Bartonella, and Hantavirus were detected in small mammals in Chongqing. These pathogens were most frequently found in R. norvegicus, R tanezumi, and A. squamipes, which were mainly distributed in the key industries of urban Chongqing and the rural residential areas of Wanzhou District, and the authorities of these places should pay more attention to the spread and control of rodent-borne diseases.
XIAO Han-sen, HE Ya-ming, TU Tao-tian, ZHANG Ying, WANG Zheng, JI Heng-qing
. Surveillance results of rodent-borne pathogens in Chongqing, China, 2021-2022[J]. Chinese Journal of Vector Biology and Control, 2023
, 34(5)
: 628
-632
.
DOI: 10.11853/j.issn.1003.8280.2023.05.008
[1] Huang YK,Chen Y,Du X,et al. Progress of rodent control[J]. Chin J Hyg Insect Equip,2015,21（6）:627-629. DOI:10.19821/j.1671-2781.2015.06.028.（in Chinese） 黄英凯,陈曜,杜雪,等. 鼠类防治研究进展[J]. 中华卫生杀虫药械,2015,21（6）:627-629. DOI:10.19821/j.1671-2781. 2015.06.028.
[2] ChenLW. Investigation on rodents and rodent-borne pathogens in Shenwan port of Zhongshan city[D]. Guangzhou:Guangdong Pharmaceutical University,2019. DOI:10.27690/d.cnki.ggdyk.2019.000192.（in Chinese） 陈利伟. 中山市神湾港区鼠形动物及其携带病原体的调查研究[D]. 广州:广东药科大学,2019. DOI:10.27690/d.cnki.ggdyk.2019.000192.
[3] Tu TT,Feng SQ,He YM,et al. An analysis of rodent surveillance results at national-level surveillance sites in Chongqing,China,2018[J]. Chin J Vector Biol Control,2019,30（6）:711-712,718. DOI:10.11853/j.issn.1003.8280.2019.06.028.（in Chinese） 涂涛田,冯绍全,何亚明,等. 重庆市2018年国家级监测点鼠类监测结果分析[J]. 中国媒介生物学及控制杂志,2019,30（6）:711-712,718. DOI:10.11853/j.issn.1003.8280.2019. 06.028.
[4] Zuo L,Tan YD,Xiong XW,et al. Rodent investigation in Wanzhou district of Chongqing from 2017 to 2021[J]. Chin J Hyg Insect Equip,2022,28（5）:431-433. DOI:10.19821/j.1671-2781.2022.05.011.（in Chinese） 左磊,谭亚东,熊学武,等. 2017－2021年重庆市万州区鼠形动物监测[J]. 中华卫生杀虫药械,2022,28（5）:431-433. DOI:10.19821/j.1671-2781.2022.05.011.
[5] Tao XY,Chen F,He JJ,et al. Investigation of the domestic rodents and infection status of rodent-borne pathogens in Shapingba district of Chongqing,China[J]. Chin J Vector Biol Control,2022,33（5）:727-731. DOI:10.11853/j.issn.1003. 8280.2022.05.021.（in Chinese） 陶晓颖,陈飞,何建军,等. 重庆市沙坪坝区家栖鼠类及鼠传病原体感染状况调查[J]. 中国媒介生物学及控制杂志,2022,33（5）:727-731. DOI:10.11853/j.issn.1003.8280.2022.05.021.
[6] Peng JY,Huang W,Ling H,et al. Monitoring analysis of rodent host of Hantavirus in Chongqing section of Three Gorges Reservoir during 2014-2016[J]. Lab Med Clin,2017,14（23）:3447-3448,3452. DOI:10.3969/j.issn.1672-9455.2017.23.009.（in Chinese） 彭靖尧,黄为,凌华,等. 三峡库区重庆段2014－2016年汉坦病毒鼠类宿主监测分析[J]. 检验医学与临床,2017,14（23）:3447-3448,3452. DOI:10.3969/j.issn.1672-9455.2017.23.009.
[7] Cheng C,Ju WD,Fu WM,et al. Epidemiologic investigation on Hantavirus carried by rodents at Heilongjiang frontier ports[J]. Chin J Zoonoses,2015,31（7）:681-686. DOI:10.3969/cjz.j.issn.1002-2694.2015.07.019.（in Chinese） 程成,鞠文东,付维明,等. 黑龙江口岸地区鼠类动物中汉坦病毒感染情况调查[J]. 中国人兽共患病学报,2015,31（7）:681-686. DOI:10.3969/cjz.j.issn.1002-2694.2015.07.019.
[8] ShangC. Study on molecular epidemiological characteristics of Hantavirus in China[D]. Beijing:Chinese Center for Disease Control and Prevention,2020. DOI:10.27511/d.cnki.gzyyy. 2020.000115.（in Chinese） 尚翠. 中国汉坦病毒分子流行病学特征研究[D]. 北京:中国疾病预防控制中心,2020. DOI:10.27511/d.cnki.gzyyy.2020. 000115.
[9] Ke XM,Wang XQ,Guo ZN,et al. Detection of Hantaviruses in rat-like animals in Haicang region,Xiamen city[J]. J Trop Med,2022,22（12）:1738-1740. DOI:10.3969/j.issn.1672-3619. 2022.12.028.（in Chinese） 柯雪梅,王秀芹,郭志南,等. 厦门市海沧区鼠形动物携带汉坦病毒调查分析[J]. 热带医学杂志,2022,22（12）:1738-1740. DOI:10.3969/j.issn.1672-3619.2022.12.028.
[10] Han YN,Liu Y,Sun QT,et al. An investigation of rodent-borne pathogens in small mammals in some areas of Shandong province,China[J]. Chin J Vector Biol Control,2022,33（6）:800-804. DOI:10.11853/j.issn.1003.8280.2022.06.006.（in Chinese） 韩英男,刘言,孙钦同,等. 山东省部分地区2020－2021年小兽携带鼠传病原体的调查[J]. 中国媒介生物学及控制杂志,2022,33（6）:800-804. DOI:10.11853/j.issn.1003.8280.2022. 06.006.
[11] Guo TY,Guo HL,Liu LJ. The review of rodent pests control in China[J]. Chin J Front Health Quar,2015,38（6）:406-408. DOI:10.16408/j.1004-9770.2015.06.007.（in Chinese） 郭天宇,郭惠琳,刘丽娟. 我国家鼠综合防治概况[J]. 中国国境卫生检疫杂志,2015,38（6）:406-408. DOI:10.16408/j.1004-9770.2015.06.007.
[12] Li ZT,Liu DX,Chen J,et al. Analysis of surveillance data on small mammals and their ectoparasites and pathogens in different port areas of Zhongshan port,China,2020-2022[J]. Chin J Vector Biol Control,2022,33（6）:793-799. DOI:10.11853/j.issn.1003.8280.2022.06.005.（in Chinese） 李植涛,刘德星,陈健,等. 2020－2022年中山口岸不同港区小型哺乳动物及其携带体表寄生虫和病原体监测分析[J]. 中国媒介生物学及控制杂志,2022,33（6）:793-799. DOI:10.11853/j.issn.1003.8280.2022.06.005.
[13] Liu WJ,Wang JX,Xiao FZ,et al. Infection status and molecular characteristics of Leptospira in rodents in northern Fujian province[J]. Chin J Zoonoses,2022,38（9）:807-812. DOI:10.3969/j.issn.1002-2694.2022.00.123.（in Chinese） 刘维俊,王加熊,肖方震,等. 福建省北部地区鼠类钩端螺旋体感染状况及分离株分子特征研究[J]. 中国人兽共患病学报,2022,38（9）:807-812. DOI:10.3969/j.issn.1002-2694.2022.00.123.
