Review

Trends in the development of methods for field detection of mosquito-borne viruses

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  • 1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
    2. Department of Pathogen Biology, Navy Medical University, Shanghai 200433, China;
    3. Department of Naval Medicine, Navy Medical University, Shanghai 200433, China

Received date: 2022-12-10

  Online published: 2023-06-16

Supported by

Municipal Natural Science Foundation of Shanghai of China (No. 19ZR1469600); National Natural Science Foundation of China (No. 31970445)

Abstract

Mosquito-borne viruses are transmitted mainly by mosquitoes during blood feeding. These viruses include more than 100 species and can cause many infectious diseases and seriously threaten human health. The accurate and rapid detection of mosquito-borne viruses in field is an important part of preventing and controlling mosquito-borne diseases. Field detection allows earlier disease prevention and control and helps control the outbreaks and epidemics of mosquito-borne diseases. However, current detection methods mainly target clinical samples and cannot be used for on-site detection of viruses in mosquito samples. This paper reviews the detection methods for mosquito-borne viruses, including the classical isolation and culture, antigen detection, and nucleic acid detection methods, as well as the new technologies developed rapidly in recent years, such as isothermal amplification and clustered regularly interspaced short palindromic repeats-associatied (CRISPR-Cas). The characteristics of these methods are analyzed, and the directions of developing technologies for monitoring mosquito-borne viruses in field are discussed.

Cite this article

JIANG Ning, MA Ya-jun, BAI Jie, PENG Heng . Trends in the development of methods for field detection of mosquito-borne viruses[J]. Chinese Journal of Vector Biology and Control, 2023 , 34(3) : 422 -427 . DOI: 10.11853/j.issn.1003.8280.2023.03.024

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