Objective To investigate the resistance level of Aedes albopictus to commonly used insecticides in Tongren of Guizhou province, China and the mechanism of metabolic resistance at the enzyme level, and to provide a theoretical basis for the control and resistance management of Ae. albopictus. Methods In August 2020, Ae. albopictus larvae were collected from small water containers in communities at different locations of Tongren and were reared to F1-F2 generations. The larval dipping method and the adult mosquito contact tube method were used to investigate the insecticide resistance of the larvae and adult mosquitoes of Ae. albopictus, and a microplate reader was used to measure the activity of non-specific esterase (NSE), mixed function oxidase (MFO), and glutathione-S-transferase (GST). SPSS 24.0 software was used to perform the statistical analysis of laboratory results, and Minitab 20 software was used to make charts. Results For the larvae of the field population of Ae. albopictus, the resistance ratios to deltamethrin, beta-cypermethrin, permethrin, temephos, and propoxur were 15.38, 7.88, 61.44, 1.70, and 1.90 folds, respectively. The 24 h mortality rates of adult mosquitoes due to deltamethrin, beta-cypermethrin, permethrin, malathion, and propoxur at diagnostic doses were 0, 58.00%, 2.13%, 79.25%, and 100%, respectively. The activities of NSE, MFO, and GST in the field population of Ae. albopictus were 1.21, 0.28, and 0.34 times, respectively, those in sensitive strains, and the activities of NSE, MFO, and GST in the field population showed a positively skewed distribution. Conclusion The Ae. albopictus larvae in Tongren have developed varying degrees of resistance to pyrethroid insecticides and remain sensitive to temephos or propoxur, while the adult mosquitoes are resistant to pyrethroid insecticides and malathion and sensitive to propoxur. Therefore, it is suggested to adopt comprehensive measures for the control of Ae. albopictus and use chemical insecticides scientifically and rationally, so as to effectively control the development and progression of insecticide resistance in Ae. albopictus.
WANG Dan, CHEN Dan-dan, TIAN Dong-dong, XU Xiu-ping, ZHOU Jing-zhu, SHI Wei-fang, LIANG Wen-qin
. Insecticide resistance and metabolic enzyme activity monitoring for Aedes albopictus in Tongren of Guizhou province, China[J]. Chinese Journal of Vector Biology and Control, 2022
, 33(6)
: 787
-792
.
DOI: 10.11853/j.issn.1003.8280.2022.06.004
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