Objective To investigate the situation of ticks and tick-borne bacteria in some areas of Guizhou province, China, and to provide a scientific basis for the prevention and control of tick-borne diseases. Methods Parasitic ticks were collected from the body surface of cattle, sheep, and rodents in Xiuwen county, Qinglong county, Xingyi city, Xingren city, Wangmo county, and Jiangkou county of Guizhou province, in April 2017 and April 2019. Morphological identification was performed on the ticks. Some ticks were selected for bacterial culture using blood agar and brain-heart infusion agar. Colonies with different morphologies were selected for bacterial passage and purification, followed by full 16S rDNA amplification and sequencing to identify the species of bacteria. Results A total of 742 on-host ticks belonging to 6 species of 3 genera were collected. Haemaphysalis longicornis was the dominant species, accounting for 69.81% of the total catches, followed by Rhipicephalus microplus, accounting for 23.05%, and the other tick species only accounted for 7.14%. A total of 188 strains of bacteria were acquired from culture, belonging to 28 genera in 3 phyla. Proteobacteria was the dominant phylum, followed by Firmicutes and Actinobacteria. Conclusion In these areas of Guizhou province, tick species are abundant, and they carry varied species of bacteria, including a variety of zoonotic pathogens. In order to prevent ticks from harming people and livestock, the investigation, monitoring, and control of ticks and tick-borne pathogens should be strengthened.
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