ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China
Received date: 2018-12-21
Online published: 2019-06-20
Supported by
Supported by the National Key Research and Development Program of Biosafety Key Technology Research and Development (No. 2016YFC1200500)
Objective To investigate the distribution of mosquito vectors and prevalence of mosquito-borne diseases in the southwest border regions of Yunnan province, China, and to provide a basis for the prevention and control of such diseases. Methods From 2013 to 2015, the methods of mosquito lamp and human landing collection were used to collect mosquitoes in Dai-Lahu-Va Autonomous County of Menglian, Menghai, and Hani-Yi Autonomous County of Jiangcheng of Yunnan. A kit was used to extract DNA and RNA, and RT-PCR and nucleotide sequencing were used to obtain the gene sequence of pathogens. Biological software including Clustal X1.83, DNASTAR 7.1, and MEGA 5.0 were used for nucleotide sequence analysis and phylogenetic analysis. Results A total of 126 893 mosquitoes of 39 species in 10 genera were collected, among which there were 104 476 Culex tritaeniorhynchus mosquitoes (82.33%), 18 422 Anopheles sinensis mosquitoes (14.52%), 1 212 Aedes albopictus mosquitoes (1.00%), and 891 Cx. pipiens quinquefasciatus mosquitoes (0.70%). Two strains of Japanese encephalitis virus (JEV) were isolated from Cx. tritaeniorhynchus, and Plasmodium, filaria, and other flaviviruses were not detected. Conclusion There are various vector mosquitoes in the border regions of Yunnan. Cx. tritaeniorhynchus and An. sinensis are dominant species in the local area. The isolated JEV suggest that the virus be prevalen in the southwest border regions of Yunnan, and therefore, the monitoring of key vector mosquitos should be enhanced.
Key words： Mosquito; Mosquito-borne disease; Pathogen; Border region; Yunnan province
XIE Lyu, LIN An-qi, WANG Jian, WU Chao, LUO Yan, YU Ai-shui, CHEN Ran, YANG Bo, XIE Xiao-lu, LI Chun-min, LI Zhi-jiao, ZHU Huai-min, JIANG Jin-yong . An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China[J]. Chinese Journal of Vector Biology and Control, 2019 , 30(3) : 264 -267 . DOI: 10.11853/j.issn.1003.8280.2019.03.008
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