Chines Journal of Vector Biology and Control ›› 2019, Vol. 30 ›› Issue (3): 264-267.DOI: 10.11853/j.issn.1003.8280.2019.03.008

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An investigation of mosquitoes and mosquito-borne pathogens in the southwest border regions of Yunnan province, China

XIE Lyu1, LIN An-qi2, WANG Jian1, WU Chao1, LUO Yan3, YU Ai-shui3, CHEN Ran4, YANG Bo5, XIE Xiao-lu5, LI Chun-min1, LI Zhi-jiao1, ZHU Huai-min2, JIANG Jin-yong1   

  1. 1 Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Academician Workstation of Professor Jin Ningyi, Yunnan Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention(Developing), Training Base of International Scientific Exchange and Education in Tropical Diseases for South and Southeast Asia, Yunnan Provincial Center of Arborvirus Research, Yunnan Institute of Parasitic Disease, Pu′er 665000, Yunnan Province, China;
    2 Department of Medical Microbiology and Parasitology, Naval Medical University;
    3 Dai-Lu-Wa Autonomous County of Menglian Center for Disease Control and Prevention;
    4 Menghai Center for Disease Control and Prevention;
    5 Hani-Yi Autonomous County of Jiangcheng Center for Disease Control and Prevention
  • Received:2018-12-21 Online:2019-06-20 Published:2019-06-20
  • Supported by:

    Supported by the National Key Research and Development Program of Biosafety Key Technology Research and Development (No. 2016YFC1200500)

云南省西南边境地区蚊虫及蚊媒病原体调查

谢吕1, 林安琪2, 王剑1, 吴超1, 罗燕3, 鱼爱水3, 陈然4, 杨波5, 谢晓露5, 李春敏1, 李志娇1, 朱淮民2, 姜进勇1   

  1. 1 云南省虫媒传染病防控研究重点实验室, 金宁一院士工作站, 云南省虫媒传染病防控关键技术创新团队(培育), 面向南亚东南亚热带病国际科技人员交流与教育培训基地, 云南省虫媒病毒研究中心, 云南省寄生虫病防治所, 云南 普洱 665000;
    2 海军军医大学病原生物学教研室, 上海 200433;
    3 孟连傣族拉祜族佤族自治县疾病预防控制中心, 云南 孟连 665800;
    4 勐海县疾病预防控制中心, 云南 勐海 666200;
    5 江城哈尼族彝族自治县疾病预防控制中心, 云南 江城 665900
  • 通讯作者: 姜进勇,Email:yipdjiang@126.com
  • 作者简介:谢吕,男,医师,主要从事虫媒病毒疾病防治工作,Email:xielv2017@outlook.com
  • 基金资助:

    国家重点研发计划生物安全关键技术研发重点专项(2016YFC1200500)

Abstract:

Objective To investigate the distribution of mosquito vectors and prevalence of mosquito-borne diseases in the southwest border regions of Yunnan province, China, and to provide a basis for the prevention and control of such diseases. Methods From 2013 to 2015, the methods of mosquito lamp and human landing collection were used to collect mosquitoes in Dai-Lahu-Va Autonomous County of Menglian, Menghai, and Hani-Yi Autonomous County of Jiangcheng of Yunnan. A kit was used to extract DNA and RNA, and RT-PCR and nucleotide sequencing were used to obtain the gene sequence of pathogens. Biological software including Clustal X1.83, DNASTAR 7.1, and MEGA 5.0 were used for nucleotide sequence analysis and phylogenetic analysis. Results A total of 126 893 mosquitoes of 39 species in 10 genera were collected, among which there were 104 476 Culex tritaeniorhynchus mosquitoes (82.33%), 18 422 Anopheles sinensis mosquitoes (14.52%), 1 212 Aedes albopictus mosquitoes (1.00%), and 891 Cx. pipiens quinquefasciatus mosquitoes (0.70%). Two strains of Japanese encephalitis virus (JEV) were isolated from Cx. tritaeniorhynchus, and Plasmodium, filaria, and other flaviviruses were not detected. Conclusion There are various vector mosquitoes in the border regions of Yunnan. Cx. tritaeniorhynchus and An. sinensis are dominant species in the local area. The isolated JEV suggest that the virus be prevalen in the southwest border regions of Yunnan, and therefore, the monitoring of key vector mosquitos should be enhanced.

Key words: Mosquito, Mosquito-borne disease, Pathogen, Border region, Yunnan province

摘要:

目的 了解云南省西南边境地区蚊媒分布及相关蚊媒传染病的存在及流行情况,为该病的预防控制提供科学依据。方法 2013-2015年在云南省孟连傣族拉祜族佤族自治县、勐海和江城哈尼族彝族自治县采用诱蚊灯法和人工诱捕法进行蚊虫采集,采用试剂盒提取蚊虫DNA及RNA,通过反转录-聚合酶链式反应和核苷酸序列测定方法获得病原体基因序列,采用Clustal X1.83、DNASTAR 7.1和MEGA 5.0等生物学软件进行核苷酸序列分析及系统进化分析。结果 共捕获成蚊10属39种126 893只,其中捕获三带喙库蚊104 476只、中华按蚊18 422只、白纹伊蚊1 212只、致倦库蚊891只,分别占82.33%、14.52%、1.00%和0.70%。分离出2株流行性乙型脑炎病毒(JEV),均来自三带喙库蚊,未检出疟原虫、丝虫及其他黄病毒。结论云南省边境地区蚊媒传染病媒介种类较多,三带喙库蚊和中华按蚊为优势蚊种,自然界中存在着JEV,应加强重要蚊媒监测工作。

关键词: 蚊虫, 蚊媒传染病, 病原体, 边境地区, 云南省

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