Objective To explore the feasibility of applying DNA barcoding technology to the classification and identification of rodent species in the plague foci in Gansu province, China. Methods In 2017, rodents were collected from the plague foci and non-plague foci in Gansu province; whole rodents and their liver were made into specimens and preserved. Genomic DNA was extracted from the rodent liver; the cytochrome C oxidase subunitⅠ(COⅠ) gene was amplified by consensus primer polymerase chain reaction, and then sequenced. The sequencing results were compared with the DNA barcodes of other rodent species in the GenBank by BLAST algorithm, and a phylogenetic tree was constructed. Results A total of 164 rodent samples were collected from four different habitats in nine cities (districts or counties) in Gansu province, including Muridae (3 species), Dipodidae (2 species), Cricetidae (4 species), Sciuridae (3 species), Gerbillidae (1 species), Spalacidae (1 species), and Ochotonidae of the Lagomorpha (1 species). The phylogenetic tree constructed from the above 164 samples formed 15 highly supportive branches, which was in accordance with the results of morphological identification. Conclusion It is feasible to apply DNA barcoding to the exact identification of rodent samples, which can facilitate the study of classification and phylogenetic relationships of rodents.
GUO Li-min, XI Jin-xiao, GAI Yong-zhi, WU Bin, WANG Shi-ming, MIAO Ke-jun, MU Tao-xia, XU Da-qin
. Application of DNA barcoding in the identification of rodent species in plague foci in Gansu province, China[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(2)
: 176
-179
.
DOI: 10.11853/j.issn.1003.8280.2019.02.014
[1] 花立民,黄倩,曹慧.甘肃啮齿动物区系组成研究[J].草原与草坪,2008(2):46-51.DOI:10.3969/j.issn.1009-5500.2008.02.010.
[2] Witt JDS,Threloff DL,Hebert PDN.DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus:implications for desert spring conservation[J].Mol Ecol,2006,15(10):3073-3082.DOI:10.1111/j.1365-294X.2006.02999.x.
[3] Hebert PDN,Ratnasingham S,de Waard JR.Barcoding animal life:cytochrome c oxidase subunit 1 divergences among closely related species[J].Proc Biol Sci,2003,270 Suppl 1:S96-99.DOI:10.1098/rsbl.2003.0025.
[4] 马英,李海龙,鲁亮,等.DNA条形码技术在青海海东地区小型兽类鉴定中的应用[J].生物多样性,2012,20(2):193-198.DOI:10.3724/SP.J.1003.2012.06188.
[5] 张涛,宁文艳,吴建华,等.宁夏啮齿动物DNA条形码分析[J].中国人兽共患病学报,2014,30(12):1234-1238.DOI:10.3969/cjz.j.issn.1002-2694.2014.12.014.
[6] 郑智民,姜志宽,陈安国.啮齿动物学[M].上海:上海交通大学出版社,2008:57-74.
[7] Kimura M.A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences[J].J Mol Evol,1980,16(2):111-120.DOI:10.1007/BF01731581.
[8] 闫东,鲁亮,金圣浩,等.DNA条形码技术在河北省鼠疫疫源地鼠种鉴定中的应用[J].中国媒介生物学及控制杂志,2014,25(1):21-23.DOI:10.11853/j.issn.1003.4692.2014.01.006.
[9] Ivanova NV,Clare EL,Borisenko AV.DNA barcoding in mammals[M]//Kress WJ,Erickson DL.DNA Barcodes:Methods and Protocols.Totowa NJ:Humana Press,2012:153-182.
[10] 张涛,段恒璐,白学礼,等.宁夏六盘山地区小型兽类DNA条形码分析[J].中国媒介生物学及控制杂志,2016,27(4):330-332.DOI:10.11853/j.issn.1003.8280.2016.04.004.
[11] 鲁亮,蒋卫,刘蓉蓉,等.中国-哈萨克斯坦边境地区啮齿动物多样性调查[J].中国媒介生物学及控制杂志,2016,27(4):317-320.DOI:10.11853/j.issn.1003.8280.2016.04.001.
[12] 王祖郧,李超.青海鼠疫[M].北京:人民卫生出版社,2016:56-61.
[13] 安翠红,陈宝宝,孙养信,等.DNA条形码技术在鼠疫疫区啮齿动物残体鉴定中的应用[J].中华地方病学杂志,2014,33(5):504-507.DOI:10.3760/cma.j.issn.2095-4255.2014.05.009.
[14] 金圣浩,鲁亮,闫东,等.DNA条形码技术在河北省坝上地区夜行鼠鉴定中的应用[J].中国媒介生物学及控制杂志,2015,26(1):55-57.DOI:10.11853/j.issn.1003.4692.2015.01.014.
[15] 程佳月,王丽华,彭克美,等.国际生命条形码计划-DNA Barcoding[J].中国畜牧兽医,2009,36(8):49-53.
