Objective To investigate the genotype and variation of hantavirus (HV) carried by host animals in the epidemic foci of hemorrhagic fever with renal syndrome (HFRS) in Shenyang, China. Methods The high-incidence areas of HFRS in Shenyang were selected. The host animals (rats) were captured simultaneously in residential areas and the field in the spring and autumn of 2017, and the rat lungs were collected. The HV antigen-positive rat lungs were detected by immunofluorescence assay (IFA), from which G2 segments were amplified by RT-PCR. Agarose gel electrophoresis was used to identify the genotype. Nucleotide sequencing of the amplified products was performed. Homology analysis was performed by DNAStar package. Results Seventeen HFRS-positive rat lung specimens were screened out by IFA. Gene segments of interest were amplified from 10 specimens by RT-PCR, all of which belonged to Seoul virus (SEO) type. The sequencing results of the amplified products indicated that, compared with domestic strains and some international standard strains, the nucleotide homology of the 10 SEO-type HV specimens was 99.3%-100%. The homology with the 80-39 strain (South Korea, 1996) was the highest (95.6%-96.2%). The deduced amino acid homology was 99.3%. Conclusion SEO was the major type of HV in HFRS epidemic foci in Shenyang. The G2 segment of HV carried by rats was highly homologous and had no obvious variation, and the genetic materials were relatively stable.
CHE Lei, ZHANG Guo-bin, GAO Dan, ZHAO Liang, WANG Xiao-li
. Genotyping and sequence analysis of G2 segment of hantavirus carried by rats in Shenyang, China[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 91
-94
.
DOI: 10.11853/j.issn.1003.8280.2019.01.022
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