Objective To analyze the expression level of transferrin gene in the susceptible population and resistant population of Culex pipiens quinquefasciatus. Methods RNA was extracted and was amplified by quantitative real-time PCR (qRT-PCR) to produce partial gene fragments of the target gene using the designed primers. After purification and sequencing, the sequence alignment was performed. The t test was used to statistically analyze the expression levels of transferrin gene in the susceptible population and resistant population of Cx. pipiens quinquefasciatus. Results The qRT-PCR analysis showed that the mean fluorescence quantification values of transferring gene in susceptible population and resistant population of Cx. pipiens quinquefasciatus were 15.21 and 18.41, respectively; the mean fluorescence quantification values of the internal reference gene in the two populations were 20.53 and 19.10, respectively. The expression level of transferrin gene in the resistant population of Cx. pipiens quinquefasciatus was reduced, and there was a significant difference in the expression level of transferrin gene between the two populations (t=10.390, P<0.001). Conclusion The transferrin gene of Cx. pipiens quinquefasciatus can be used as a target gene for the detection and treatment of mosquito resistance.
YANG Hui-na, LU Jing, TAN Wen-bin
. An analysis of transferrin gene expression in resistant population and susceptible population of Culex pipiens quinquefasciatus[J]. Chinese Journal of Vector Biology and Control, 2019
, 30(1)
: 50
-55
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DOI: 10.11853/j.issn.1003.8280.2019.01.011
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