Objective To observe the growth of Yersinia pestis on different kinds of culture media and screen for high quality culture media. Methods Make 5 kinds of culture media (Hiss Agar, Nutrient Agar, Sulfite Agar base, Hemolysis Agar Base, and Agar Hottinger nutrient)according to the instructions and pour into plates. Inoculating 0.1 ml Y. pestis suspensions on the prepared plate, calculating viable bacteria after 48-hour cultivation under 28℃ and observing the growth of Y. pestis on different culture media. Results There is no significant difference among Hiss Agar, Nutrient Agar and Sulfite Agar base. And the difference between Hemolysis Agar Base and Agar Hottinger nutrient was not significant either. The first three culture media and the following two culture media were also not significantly different. Hemolysis Agar Base and Agar Hottinger nutrient were superior to other culture media Hiss Agar, Nutrient Agar, Sulfite Agar base on growth of Y. pestis. Conclusion All the five culture media can be used for culture of Y. pestis, but Hemolysis Agar Base and Agar Hottinger nutrient were superior to the others.
FENG Jian-ping, GUO Wen-tao, YANG Jian-guo, LI Cun-xiang, ZHAO Hai-hong, JIN Xing, XIN You-quan, JIN Li-xia, XIONG Hao-ming, QI Zhi-zhen
. Evaluation on the growth of Yersinia pestis on 5 kinds of culture medias[J]. Chinese Journal of Vector Biology and Control, 2018
, 29(3)
: 303
-304
.
DOI: 10.11853/j.issn.1003.8280.2018.03.022
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