Objective To investigate the species and fauna of the flies in Shenzhen city. Methods During 2010-2015, sweeping net and fly-trap were used to collect flies in Shenzhen city, specimens were identified to establish species list, historical documents were collected to analysis fauna. Results A total of 77 species of flies were obtained from Shenzhen city (38 genera, 3 families). Five species were first collected in Guangdong province, 22 species were new recorded to Shenzhen city. There were 25 oriental species, 13 oriental and palearctic shared species, 12 oriental, palearctic and Australian shared species, 27 the rest groups species. There were 18 southern China species, 14 central and southern China shared species, 11 widespread species, 7 widespread (without Qinghai Tibet region)species, 27 the rest groups species. Conclusion The fauna of flies in Shenzhen city is complex, groups in the Oriental Region are distinctly predominant, southern China species and central-southern China shared species constitute most fly species in Shenzhen city.