Objective To detect Bartonella spp. nucleic acid from main ectoparasite fleas in rodents and free-living ticks from Alashankou pass, China-Kazakhstan border. Methods Collected fleas and ticks were then identified by morphological features. After species identification, genome DNA was extracted and detected by PCR targeting gltA, ITS and ribC of Bartonella spp. The amplified products were sequenced and the sequences analyzed using the Blast. A phylogenetic tree was constructed using Mega 6.0 software. Results A total 2 139 fleas (7 species, 6 genera) were identified. Xenopsylla minax was the predominant species. A total of 243 free-living ticks were identified as Hyalomma asiaticum. Only X. minax, Coptospllya lamellifer ardua, and Parddoxopsyllus repandustested positive for Bartonella spp. and all the ticks were negative. Sequence analysis showed that three different sequences, DNAman homology were 93.61%, 52.54%, and 86.72%, respective, gltA, ITS, and ribC. Blast and phylogenetic analyses showed that this three Bartonella and Bartonella sp. AL01,02, 04 were same sequence. Conclusion Bartonella nucleic acid was detected in three different wild rat-flea species at Alashankou pass.
YIN Xiao-ping, ZHAO Shan-shan, TIAN Yan-he, BA Te, ZHANG Jiang-guo, CHENG Tian-li, WANG Yuan-zhi
. Detection of Bartonella from rodent-harboring fleas and free-living ticks at Alashankou pass,China-Kazakhstan border[J]. Chinese Journal of Vector Biology and Control, 2017
, 28(4)
: 350
-353
.
DOI: 10.11853/j.issn.1003.8280.2017.04.011
[1] 栗冬梅,刘起勇,俞东征,等. 蚤、蜱中巴尔通体的分离培养及检测鉴定[J]. 中国人兽共患病杂志,2005,21(12):1052-1058,1074.
[2] Pérez-Martínez L, Venzal JM, Gonz——lez-Acu-a D, et al. Bartonella rochalimae and other Bartonella spp. in fleas,Chile[J]. Emerg Infect Dis,2009,15(7):1150-1152.
[3] Liu QY, Eremeeva ME, Li DM. Bartonella and Bartonella infections in China:from the clinic to the laboratory[J]. Comp Immunol Microbiol Infect Dis,2012,35(2):93-102.
[4] 尹小平,彭定希,李东,等. 阿拉山口口岸地区蚤类区系调查及动态分析[J]. 地方病通报,2008,23(1):33-36.
[5] 尹小平,叶志辉,骄娃,等. 阿拉山口口岸地区蜱类区系调查及宿主动物染带关系监测分析[J]. 中国媒介生物学及控制杂志,2010,21(4):375-377.
[6] Johnson G, Ayers M, McClure SCC, et al. Detection and identification of Bartonella species pathogenic for humans by PCR amplification targeting the riboflavin synthase gene (ribC)[J]. J Clin Microbiol,2003,41(3):1069-1072.
[7] 栗冬梅,刘起勇,俞东征,等. 用PCR方法检出蚤类携带巴尔通体[J]. 中国媒介生物学及控制杂志,2005,16(1):5-8.
[8] 尹小平,叶志辉,彭定希,等. 臀突客蚤重要生态学调查与流行病学意义的探讨[J]. 中国媒介生物学及控制杂志,2011,22(2):124-127.
[9] 尹小平,叶志辉,叶瑞玉,等. 臀突客蚤的生活史及其叮吸人血试验[J]. 中国媒介生物学及控制杂志,2010,21(1):61-62.
