Chines Journal of Vector Biology and Control ›› 2017, Vol. 28 ›› Issue (4): 350-353.DOI: 10.11853/j.issn.1003.8280.2017.04.011

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Detection of Bartonella from rodent-harboring fleas and free-living ticks at Alashankou pass,China-Kazakhstan border

YIN Xiao-ping1, ZHAO Shan-shan2, TIAN Yan-he1, BA Te1, ZHANG Jiang-guo1, CHENG Tian-li1, WANG Yuan-zhi2   

  1. 1 Alashankou Entry-Exit Inspection and Quarantine Bureau, Alashankou 833418, Xinjiang Uygur Autonomous Region, China;
    2 College of Animal Science and Technology, Shihezi University
  • Received:2017-04-27 Online:2017-08-20 Published:2017-08-20
  • Supported by:
    Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject (No. 2016IK264)

中国-哈萨克斯坦边境阿拉山口口岸鼠体蚤及游离蜱感染巴尔通体调查

尹小平1, 赵姗姗2, 田延河1, 巴特1, 张江国1, 程天利1, 王远志2   

  1. 1 阿拉山口出入境检验检疫局, 新疆 阿拉山口 833418;
    2 石河子大学动物科技学院, 新疆 石河子 832000
  • 通讯作者: 王远志,Email:wangyuanzhi621@126.com
  • 作者简介:尹小平,男,副研究员,主要从事口岸医学媒介监测工作,Email:yxpciq@163.com
  • 基金资助:
    国家质检总局科技计划项目(2016IK264)

Abstract: Objective To detect Bartonella spp. nucleic acid from main ectoparasite fleas in rodents and free-living ticks from Alashankou pass, China-Kazakhstan border. Methods Collected fleas and ticks were then identified by morphological features. After species identification, genome DNA was extracted and detected by PCR targeting gltA, ITS and ribC of Bartonella spp. The amplified products were sequenced and the sequences analyzed using the Blast. A phylogenetic tree was constructed using Mega 6.0 software. Results A total 2 139 fleas (7 species, 6 genera) were identified. Xenopsylla minax was the predominant species. A total of 243 free-living ticks were identified as Hyalomma asiaticum. Only X. minax, Coptospllya lamellifer ardua, and Parddoxopsyllus repandustested positive for Bartonella spp. and all the ticks were negative. Sequence analysis showed that three different sequences, DNAman homology were 93.61%, 52.54%, and 86.72%, respective, gltA, ITS, and ribC. Blast and phylogenetic analyses showed that this three Bartonella and Bartonella sp. AL01,02, 04 were same sequence. Conclusion Bartonella nucleic acid was detected in three different wild rat-flea species at Alashankou pass.

Key words: Bartonella, Flea, Tick, Polymerase chain reaction, Alashankou pass, China-Kazakhstan border

摘要: 目的 对中国-哈萨克斯坦(中哈)边境阿拉山口口岸地区捕获的野生鼠类体表寄生蚤与游离蜱进行巴尔通体核酸检测,为该地区鼠传疾病提供风险预警和依据。方法 于2016年1-9月采集鼠类体表蚤及游离蜱,进行形态学鉴定,提取蚤类全基因组,采用PCR扩增巴尔通体gltAITSribC基因,阳性产物进行Blast分析,利用Mega 6.0软件构建分子遗传进化树。结果 共采集蚤2 139匹,隶属于6属7种,其中臀突客蚤为优势蚤种;采集游离蜱243只,均为亚洲璃眼蜱。其中臀突客蚤、叶状切唇蚤突高亚种和后弯怪蚤均检测到巴尔通体DNA片段,蜱中未检测到巴尔通体阳性片段。通过测序得到3株不同基因的巴尔通体序列,gltAITSribC的DNAman同源性分别为93.61%、52.54%和86.72%。Blast比对和进化树分析结果与Bartonella sp.AL01、02、04相同。结论 中哈边境阿拉山口口岸地区3种鼠体寄生蚤均携带巴尔通体。

关键词: 巴尔通体, 蚤, 蜱, 聚合酶链式反应, 阿拉山口口岸, 中国-哈萨克斯坦边境

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