Objective To investigate the susceptibility of Aedes albopictus larvae to four insecticides in some areas of Chongqing. Methods To determine the median lethal concentration (LC⁵⁰) of the field population of Ae. albopictus larvae of Yuzhong district and Wanzhou district located in the hinterland of the Three Gorges Reservoir, by immersing them with pesticide in 2014-2015. Results In comparison with the susceptible strain, the resistance ratios of Ae. albopictus larvae to beta-cypermethrin, deltamethrin, permethrin, and temephos were 7.59-, 4.50-, 9.88-, and 3.18-fold respectively in Yuzhong district, and 12.82-, 7.50-, 13.29-, and 2.00-fold respectively in Wanzhou. Conclusion Aedes albopictus larvae of Yuzhong had a low level resistance to beta-cypermethrin, deltamethrin, permethrin, and temephos; that of Wanzhou had a moderate resistance to beta-cypermethrin and permethrin, but a low level resistance to deltamethrin. Therefore, it is important to rotate pesticides and choose appropriate doses in mosquito control.