ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Chinese Journal of Vector Biology and Control >
Application of DNA barcoding in classification of rodents at Alataw pass, China-Kazakhstan
Received date: 2016-04-15
Online published: 2016-10-20
Supported by
Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject (No. 2016IK264)
Objective To evaluate the cytochrome C oxidase subunit Ⅰ(COⅠ) and cytochrome b (Cytb) gene as the DNA barcodes for identifying the rodent species in the Alataw pass, China-Kazakhatan. Methods The genome DNA were extracted from rodent samples of 7 species collected from Alataw pass, China-Kazakhatan during the 2013-2014. The mitochondrial COⅠand Cytb genes was amplified by PCR and sequenced. The phylogenetic trees were constructed by Mega 6.0 based on the nucleotide sequences of rodents published in the GenBank and the homology was analyzed. Results The 382 rodent samples of 7 species were clustered into 4 genera during the 2013-2014. The identification of COⅠ and Cytb gene results were 100% matched with the morphological analysis. Conclusion DNA barcoding can be used to effectively identify rodent species. DNA barcode technology provides a quick and accurate tool to identify the pathogen hosts and exotic medical vectors.
WANG An-dong, YIN Xiao-ping, TIAN Yan-he, LIANG Zhen, BA Te, ZHANG Jiang-guo . Application of DNA barcoding in classification of rodents at Alataw pass, China-Kazakhstan[J]. Chinese Journal of Vector Biology and Control, 2016 , 27(5) : 459 -462 . DOI: 10.11853/j.issn.1003.8280.2016.05.009
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